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2003 Fiscal Year Final Research Report Summary

Study on macrophage inactivation factors derived from head and neck cancers

Research Project

Project/Area Number 13470445
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionMatsumoto Dental University

Principal Investigator

YAMAOKA Minoru  Matsumoto Dental University, School of Dentistry, Professor, 歯学部, 教授 (50064671)

Co-Investigator(Kenkyū-buntansha) MATSUURA Takashi  Matsumoto Dental University, School of Dentistry, Assistant, 歯学部, 助手 (10298408)
TANAKA Hitoshi  Matsumoto Dental University, School of Dentistry, Assistant Professor, 歯学部, 講師 (50267788)
UEMATSU Takashi  Matsumoto Dental University, Graduate School of Oral Medicine, Associate Professor, 大学院・歯学独立研究科, 助教授 (40203476)
Project Period (FY) 2001 – 2003
Keywordsmacrophage activating factor (GcMAF) / a-N-acetylgalactosaminidase / head and neck cancer / cellular immunodeficiency / salivary gland adenocarcinoma
Research Abstract

The present study aimed to clarify whether human salivary gland adenocarcinoma cell-derived a-N-acetylgalactosaminidase (a-NaGalase) affects the bioactivity of an Ο-linked immune-related biomacromolecule, macrophage-activating factor (GcMAF). Compared to the squamous cell carcinoma cell line, SCCTM, high levels of exo-a-NaGalase activity were detected in cell extract and conditioning medium of the human salivary gland cell adenocarcinoma cell line HSG. Normal keratinocytes and fibroblasts exhibited low levels of exo-a-NaGalase activity. A protein from HSG was purified by ion exchange and gel filtration chromatography using exo-a-NaGalase activity assay as an indicator of fractionation. A characteristic study of the purified enzyme indicated that the enzyme hydrolyzes synthetic substrates and displays low intrinsic endo-a-NaGalase and a-galactosidase activities in addition to exo-a-NaGalase activity. Monocytes/macrophages incubated with HSG-derived a-NaGalase-treated GcMAF in the superoxide generation assay exhibited decreased superoxide-generation capacity compared to cells incubated with GcMAF alone. Phagocytic activity of monocytes/macrophages was increased by incubation with GcMAF, but not by HSG-derived a-NaGalase-treated GcMAF. In vitro deglycosylation of Gc protein using peanut agglutinin lectin, which recognizes galactose/N-acetylgalactosamine residues, demonstrated that HSG-derived a-NaGalase displays both exo-and endo-enzyme activities and facilitates sialidase activity. These results demonstrate that HSG-derived a-NaGalase possesses unique enzymatic properties compared to the constitutive enzyme of normal cells, and represents a candidate cellular immunodeficiency factor in the GcMAF related-immune cascade for host defense in cancer patients with salivary gland adenocarcinomas.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Uematsu T.: "Multidrug resistance gene 1 expression in salivary gland"International Journal of Cancer. 92. 187-194 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uematsu T.: "P-glycoprotein expression in the major and minor salivary glands"Archive of Oral Biology. 46. 521-527 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaoka M.: "Relationship between third molar development and root angulations"J.Oral Rehabil. 28. 198-205 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaoka M.: "Alteration of the angle of the coronoid process in prognathism"J.Oral Rehabil. 28. 479-484 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaoka M.: "Relationship of the hyoid bone and posterior surface of the tongue in prognathism and macrognathia"Journal of Oral Rehabilitation. 30. 914-920 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uematsu T.: "Expression of ATP-binding cassette transporter in human salivary ducts."Archive of Oral Biology. 48. 87-90 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuura T.: "Effect of salivary gland adenocaricinoma cell-derived alpha-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor."Int J Oncol.. 24. 521-528 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uematsu T.: "Effects of oral squamous cell-derived TGFβ1on CD26/DPPIV in T cells."Anticancer Research. 24. 619-624 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiba T.: "Effect of inorganic polyphosphate on periodontal regeneration"Key Engineering Material. 245-256. 1119-1122 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uematsu T.: "Multidrug resistance gene 1 expression in salivary gland adenocarcinomas and oral squamous cell carcinomas"International Journal of Cancer. Vol.92 No.2. 187-194 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uematsu T.: "P-glycoprotein expression in the major and minor salivary glands"Archive of Oral Biology. Vol.46 No.6. 521-527 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaoka M.: "Relationship between third molar development and root angulations"Journal of Oral Rehabilitation. Vol.28. 198-205 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaoka M.: "Alteration of the angle of the coronoid process in prognathism"Journal of Oral Rehabilitation. Vol.28. 479-484 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaoka M.: "Relationship of the hyoid bone and posterior surface of the tongue in prognathism and macrognathia"Journal of Oral Rehabilitation. Vol.30 No. 9. 914-920 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uematsu T.: "Expression of ATP-binding cassette transporter in human salivary ducts."Archive of Oral Biology. Vol.48 No.1. 87-90 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuura T.: "Effect of salivary gland adenocarcinoma cell-derived alpha-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor."International Journal of Oncology. Vol.24. No.3. 521-528 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uematsu T.: "Effects of oral squamous cell-derived TGF-β1on CD26/DPPIV in T cells."Anticancer Research. Vol.24. 619-624 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiba T.: "Effect of inorganic polyphosphate on periodontal regeneration"Key Engineering Material. Vol.245-256. 1119-1122 (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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