2003 Fiscal Year Final Research Report Summary
Multinuclear giant cell formation mechanism of Krabbe disease patient brain
| Project/Area Number |
13470488
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
KOZUTSUMI Yasunori Kyoto University, Graduate school of Biostudies, Professor, 生命科学研究科, 教授 (70205425)
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| Project Period (FY) |
2001 – 2003
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| Keywords | krabbe disease / Globoid cell / multinuclear cell / psychosine / tryptophan / yeast / organelle / sphingolipid |
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| Research Abstract |
The goal of this project is to understand the mechanism underlining multinuclear cell formation by psychosine. The following findings were obtained during 3years.
(1) Multinuclear giant cell formation mechanism by Psychosine
Psychosine inhibited cytokinesis and induced multinuclear cells by disrupting membrane transport to the cleavage furrow during cytokinesis.
(2) Abnormal distribution of intracellular organelles by psychosine.
Psychosine induced selective dispersal of lysosomes and earl endosomes without affecting the endocytosis activity of the cells.
(3) Genes involved in resistance to psychosine in yeast
Psychosine resistant genes were isolated using yeast system. One of genes encodes tryptophan permease. Tryptophan permease was degraded by the treatment of psychosine via the Golgi-vacuole membrane transport system.
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