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2002 Fiscal Year Final Research Report Summary

Effects of diesel exhaust particles on cardiorespiratory health

Research Project

Project/Area Number 13480173
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

TAKANO Hirohisa  National Institute for Environmental Studies, Team Leader, 環境ホルモン・ダイオキシン研究プロジェクト, 総合研究官 (60281698)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Toshikaju  Kyoto Prefectural University of Medicine, Professor, 医学部, 教授 (90158410)
ICHINOSE Takamichi  Oita University for Nursing and Health Sciense, Professor, 看護学部, 教授 (50124334)
Project Period (FY) 2001 – 2002
Keywordsdiesel exhaust particles / particulate matter / nitrogen dioxide / lung injury / Circulatory disturbance / infection / bronchial asthoma / (aortic sclerosis) atherosclerosis
Research Abstract

Epidemiology studies demonstrate acute and serious adverse effects of particulate air pollution on cardiorespiratory health especially in the predisposed people susceptible to bacterial infection. We determined the effects of diesel exhaust particles (DEP), major participants in particulate pollutants, on lung injury related to bacterial endotoxin in mice. Intratracheal instillation of DEP synergistically enhanced lung injury related to endotoxin from gram-negative bacteria, which was characterized by neutrophil sequestration, interstitial edema, and alveolar hemorrhage. In the presence of endotoxin, DEP further activated the nuclear translocation of p65 subunit of NF-κ B in the lung, and increased the lung expression of ICAM-1, IL-1 β , macrophage chemoattractant protein-1, KC, macrophage inflammatory protein-1 α , and Toll-like receptors. DEP given alone increased the lung expression of Toll-like receptor 4 and the nuclear localization of p50 subunit of NF-κ B. These results provide the first experimental evidence that DEP enhance neutrophilic lung inflammation related to bacterial endotoxm. The enhancement should be mediated by the induction of proinflammatory molecules likely through the expression of Toll-like receptors and the activation of p65-containing dimer(s) of NF-κ B such as p65/p50 (reference 5).
Furthermore, we have shown DEP or DE enhance airway inflammation related to house dust mite allergen possibly via the enhanced expression of IL-5 and EOTAXIN (reference 6, 9). In another series of experiments, we have demonstrated that DE enhances both immediate and late airway responses as well as mucus secretion (reference 1). In addition, DEP can affect immune systems via the modification of oral tolerance or Th1/Th2 balance (reference 2, 3). Finally, we have demonstrated that nitrogen dioxide in DE can enhance atherogenic metabolic complications especially in obese subjects (submitted).

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Hashimoto K, Takano H, et al.: "Exposure to diesel exhaust excerbates allergen-induced airway responses in guine a pigs"American Journal of Respiratory and Critical Care Medicine. 164. 1957-1963 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshino S, Takano H, et al.: "Effect of diesel exhaust particle extracts on induction of oral tolerance in mice"Toxicological Sciences. 66. 293-297 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] Takano H, et al.: "Diesel Exhaust Particles Enhance Lung Injury Related to Bacterial Endotoxin through Expression of Proinflammatory Cytokines, Chemokines, and ICAM-1"Americal Journal of Respiratory and Critical Care Medicine. 165. 1329-1335 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] Takano H, et al.: "Lung expression of cytochrome P450 as a possible biomarker of exposure to diesel exhaust particles"Archines of Toxicology. 76. 146-151 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] Sadakane K, Takano H, et al.: "Marine strain differences in airway inflammation indned by diesel exhaust particles and house dust mite allergen"International Archines of Allergy and Immunology. 128. 220-228 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] Ichinose T, Takano H, et al.: "Differences in airway-inflammation development by house dust mice and diesel exhaust inhalation among mouse strains"Toxicol and Applied Pharmacology. (in press).

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      「研究成果報告書概要(和文)」より
  • [Publications] 高野 裕久 他: "「ビタミンEと大気汚染」 ビタミンEの臨床"医薬ジャーナル社(印刷中).

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      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto K, Ishii Y, Uchida Y, Kimura T, Masuyama K, Morishima Y, Hirano K, Nomura A, Sakamoto T, Takano H, Sagai M, Sekizawa K: "Exposure to diesel exhaust exacerbates allergen-induced asthmatic responses in guinea pigs"Am J Respir Crit Care Med. 164. 1957-1963 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshino S, Hayashi H, Taneda S, Takano H, Sagai M, Mori Y: "Effects of diesel exhaust particle extracts on TH1 and TH2 immune response in mice"Int J Immunopathol Pharmacol. 15. 13-18 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshino S, Hayashi H, Taneda S, Takano H, Sagai M, Mori Y: "Effect of diesel exhaust particle extracts on induction of oral tolerance in mice"Toxicol Sci. 66. 293-297 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Takano H, Yanagisawa K, ichnose T, Sadakane K, Inoue K, Yoshida S, Takeda K, Yoshino S, Yoshikawa T, Morita M: "Lung expression of cytchrome P450 1A1 as a possible biomarker of exposure to diesel exhaust particles"Arch Toxicol. 76. 146-151 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Takano H, Yanagisawa R, Ichinose T, Sadakane K,Yeshino S, Yoshikawa T, Morita M: "Diesel exhaust particles enhance lung injury related to bacterial endotoxin through expression of proinflammatory cytokines, chemokines, and ICAM-1"Am J Respir Crit Care Med. 165. 1329-1335 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sadakane K, Ichinose T, Takano H, Yanagisawa R, Sagai M, Yoshikawa T, Shibamoto T: "Murine strain differences in airway inflammation induced by Diesel exhaust particles and house dust mite allergen"Int Arch Allergy Immunol. 128. 220-228 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ichinose T, Takano H, Miyabara Y, Sagai M, Sadakane K, Shibamoto T: "Diesels exhaust particles enhance antigen-induced eosinophilic inflammation in the airway of mast cell deficient mice"Toxicology. 180. 293-301 (2002)

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  • [Publications] Sadakane K, Takano H, Ichinose T, Yanagisawa R, Shibamoto T: "Formaldehyde enhances mite allergen-induced eosinophilic inflammation in the murine airway"J Environmental Pathology Toxicology and Oncology. 21. 267-276 (2002)

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  • [Publications] Ichinose T, Takano H, Sadakane K, Yanagisawa R, Kawazato H, Sagai M, Shibamoto T: "Differences in airway-inflammation development by house dust mite and diesel exhaust inhalation among mouse strains"Toxicol Applied Pharmacol. (in press).

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      「研究成果報告書概要(欧文)」より
  • [Publications] Sanbongi C, Takano H, Osakabe N, Sasa N, Natsume M, Yanagisawa R, Inoue K, Yoshikawa T: "Rosmarinic acid inhabits lung injury induced by diesel exhaust particles"Free Radic Biol Med. (in press).

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      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue K, Takano H, Yoshikawa T: "Inflammatory chemokine in the pathogenesis of the common cold"Lancet. (in press).

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      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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