2002 Fiscal Year Final Research Report Summary
Studies on the function of a centrosome and Golgi localized giant anchoring protein CG-NAP
| Project/Area Number |
13480209
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kobe University |
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Principal Investigator |
ONO Yoshitaka Kobe University, Biosignal Research Center, Professor, バイオシグナル研究センター, 教授 (10243297)
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| Project Period (FY) |
2001 – 2002
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| Keywords | protein kinases / PKN / PKC / anchoring proteins / CG-NAP / centrosome / Golgi apparatus |
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| Research Abstract |
Protein kinases are the key components in the intracellular signal transduction. The signaling specificity of the protein kinases may be mediated by the targeting of them to the specific micro-organelles through the anchoring proteins. CG-NAP (centrosome and Golgi localized PKN-associated protein) is a coiled-coil protein identified as a binding protein for the regulatory domain of PKN that has a catalytic domain highly homologous to PKC in the carboxyl-terminal region and a unique regulatory domain in the amino-terminal region. In this study, we have analyzed the function of CG-NAP as an anchoring protein and obtained the results as follows.
1. CG-NAP is a 450-kDa coiled-coil protein and is localized to centrosome throughout the cell cycle, the midbody at telophase, and the Golgi apparatus at interphase.
2. In addition to PKN, CG-NAP associates with protein kinase A, PKCε, and protein phosphatases (PP1 and PP2A).
3. CG-NAP anchors non/hypo-phosphrylated PKCε at Golgi/centrosome area during maturation, and serves as a scaffold for the phosphorylation reaction.
4. CG-NAP provides sites for microtubule nucleation in the mammalian centrosome by anchoring γ-tubulin ring complex.
5. The carboxyl-terminal region of CG-NAP is necessary for the localization to centrosome.
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