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2003 Fiscal Year Final Research Report Summary

The development of artifical cell motility and analysis of 3D motility

Research Project

Project/Area Number 13480213
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

HIGUCHI Hideo  Tohoku University, Graduate school of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (90165093)

Project Period (FY) 2001 – 2003
Keywordskinesin / motor protein / single molecules / micro scope
Research Abstract

Movement is a fundamental charlatanistic of all living things. This is a biogenic function that is attributed to the molecular motors such as kinesin, dynein and myosin. Molecular motors generate forces by using chemical energy derived from the hydrolysis reaction of ATP molecules. Despite a large number of studies on this topic, the chemomechanical energy transduction mechanism still remains unsolved. In this study, the chemomechanical coupling of the ATPase cycle to the mechanical events has been examined for the molecular motor kinesin using single molecule detection (SMD) techniques. The SMD techniques allowed the detection of the movement of single kinesin molecules along a microtubule and showed that kinesin moves in a stepwise manner. Kinesin steps mainly in the forward direction, but occasionally in the backward direction, indicating that the stepping motion is determined by stochastic processes. The stepwise movement is essentially driven by Brownian motion biased in the forward direction and is caused by the interaction between kinesin and the microtubule, which can be dynamically changed by the free energy of ATP hydrolysis.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Hideo Higuchi: "Alternate fast and slow stepping of a heterodimeric kinesin molecule."Nature Cell Biol. 5. 1079-1082 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideo Higuchi: "Single-molecule imaging of cooperative assembly of gamma-hemolysin on erythrocyte membraues."EMBO.J.. 22. 4968-4979 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideo Higuchi: "Processivity of the single-headed kinesin KIF1A through biased binding to tubalin."Nature. 424. 574-577 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideo Higuchi: "Coordination of kinesin's two heads studied with mutant heterodimer."Proc.Natl.Acad.Sci.. 96. 18058-18063 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideo Higuchi: "Controlling pore assembly of staphylococcal g-haemolysin by low temperature and by disulphide bond formation in double-cysteine LukF mutants."Mol.Microbiol.. 45. 1485-1498 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideo Higuchi: "Chemomechanical coupling of the forward and backward steps of single kinesin molecules."Nature Cell Biol.. 4. 790-797 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaseda, K, H.Higuchi, K.Hirose: "Alternate fast and slow stepping of a heterodimeric kinesin molecule."Nature cell Biol.. 5. 1079-1082 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nguyen, V.T., Y.Kamio, H.Higuchi: "Single-molecule imaging of cooperative assembly of gamma-hemolysin on erythrocyte membranes."EMBO.J.. 22. 4968-4979 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada, Y, H.Higuchi, N.Hirokawa: "procesivity of the single-headed kinesin KIFIA through biased binding to tubulin."Nature. 424. 574-577 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaseda, K, H.Higuchi, K.Hirose: "Coordination of kinesin's two heads sutudied with mutant heterodimer."Proc.Natl.Acad.Sci.. 96. 18058-18063 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nguyen, V.T., Higuchi, H., Kamio Y.: "Controlling pore assembly of staphylococcal g-hemolysin by low temperature and by disulfide bond formation in double-cystein LukF mutants"Mol.Microbiol.. 45. 1485-1498 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishiyama, M., H.Higuchi, T.Yanagida: "Chemomechanical coupling of the forward and backward steps of single kinesin molecules"Nature Cell Biol.. 4. 790-797 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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