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2003 Fiscal Year Final Research Report Summary

Analysis of the molecular mechanism of the novel topological transformation of membrane vesicle and the construction of the cell-model

Research Project

Project/Area Number 13480218
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionNagoya University

Principal Investigator

HOTANI Hirokazu  Nagoya University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (80025444)

Co-Investigator(Kenkyū-buntansha) TAKIGUCHI Kingo  Nagoya University, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 助手 (20262842)
Project Period (FY) 2001 – 2003
KeywordsOrtical Tweezers / Lirosome / Cytoskeleton / Artificial Cell
Research Abstract

(1) Liposome manipulation. The morphologies of living cells are based on the mechanical characteristics of cytoskeleton and membrane. To investigate the role of lipid membrane in the morphogenesis, we manipulated the giant liposomes that encapsulated two polystyrene beads by using double beam laser tweezers. As mechanical force was applied onto the beads to push a liposome membrane from inside, a spherical liposome transformed into a lemon shape with increasing the tension, and subsequently a tubular membrane projection was generated at the end of the lemon-shaped liposome. In an elongation stage of the lemon shape, the force required for the transformation became larger. Just before a membrane tube was generated, the force reached the maximum strength. However, after the membrane tube was developed, the force suddenly decreased, and kept constant strength independently from the tube length. These results indicate that lipid membrane can form the membrane tube by simple applying of mechanical force.
(2) Cell model developed with giant liposome. It is well established that many kinds of actin binding proteins play regulatory roles in the organization of actin networks in living cells. To study such organization roles, we characterized the transformation of liposomes encapsulating actin with its crosslinking proteins, fascin, a-actinin or filamin. As the actin polymerized, which caused various morphological changes of liposomes. The differences in morphology indicate that the actin-crosslinking proteins actually determine liposome shape by organizing their specific actin networks.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] F.Nomura: "Capabilities of liposomes for topological transformation."Proceedings of the National Academy of Sciences USA.. 98. 2340-2345 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] F.Nomura: "Morphological and Topological Transformation of Membrane Vesicles."Journal of Biological Physics. 28. 225-235 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Hotani: "Morphological and Topological Transformation of Liposomes."New Approaches to Structural Mechanics, Shells and Biological Structures. 435-446 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Fukata: "CRMP-2 Binds to Tubulin Heterodimers to Promote Microtubule Assembly."Nature Cell Biology. 4. 583-591 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Hotani: "Mechanical Analyses of Morphological and Topological Transformation of Liposomes."BioSystems. 71. 93-100 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] F.Nomura: "Microscopic Observations Reveal that Fusogenic Peptides Induce Liposome Shrinkage Prior to Membrane Fusion."Proceedings of the National Academy of Sciences USA.. 101. 3240-3425 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NOMURA, F.et al.: "Capability of liposomes for topological transformation."Proc.Natl.Acad.Sci.USA. 98. 2340-2345 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NOMURA, F.et al.: "Morphological and topological transformation of membrane vesicles."J.Biol.Phys.. 28. 225-235 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HOTANI, H., et al.: "Morphological and topological transformation of liposomes."New Approaches to Structural Mechanics, Shells and Biological Structure. 435-446 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] FUKATA, Y.et al.: "CRMP-2 binds to tubulin heterodimers to promote microtubule assembly."Nature Cell Biology. 4. 583-591 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HOTANI, H., et al.: "Mechanical analysis of morphological and topological transformation of liposomes."Biosystems. 71. 93-100 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NOMURA, F., et al.: "Microscopic observation reveal that fusogenic peptides induce liposome shrinkage prior to membrane fusion."Proc.Natl.Acad.Sci.USA. 101. 3420-3425 (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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