2002 Fiscal Year Final Research Report Summary
Analysis of the function of CD9 and tetraspanins
| Project/Area Number |
13480238
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
MEKADA Eisuke Osaka University, Research Institute for Microbial Diseases, Professor, 微生物病研究所, 教授 (20135742)
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| Co-Investigator(Kenkyū-buntansha) |
MIYADO Kenji Osaka University, Research Institute for Microbial Diseases, Research Associate, 微生物病研究所, 助手 (60324844)
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| Project Period (FY) |
2001 – 2002
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| Keywords | membrane fusion / C. elegans / fertilization / epidermis / RNAi / complex |
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| Research Abstract |
(1) CD9-/-eggs are defect in fertilization, especially the fusion step with sperm.The fusion activity of CD9-/-eggs with sperm was recovered by the injection of CD9 mRNA into CD9-/-eggs. We constructed various mutant forms of CD9 mutants and examined their activities by injection their mRNA to CD9-/-eggs. These experiments suggestrd that the C-terminal domain of CD9 is essential for the recovery of fusion activity.
(2) There are 29 members of the tetraspanins in C. elegans. We carried out RNA interference experiments for these tetraspanins to explore the role of tetraspanins in a whole animal. One of the tetraspanins, TSP-15, showed marked effect in body integrity. Present study opens new approach to analyse tetraspanins function by genetic methods.
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[Publications] Umata, T. Hirata, M. Takahashi, T. Ryu, F. Shida, S. Takahashi, Y. Tsuneoka, M. Miura, Y. Masuda, M. Horiguchi, Y. and Mekada, E.: "A dual signaling cascade that regulates the ectodomain shedding of HB-EGF."J. Biol. Chem. 276. 30475-30482 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Saito, M. Iwawaki, T. Taya, C. Yonekawa, H. Munehiro, N. Inui, Y. Mekada, E.. Kimata, Y. Tsuru, A. and Kohno, K..: "Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice."Nat. Biotechnol.. 19. 746-750 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Shimizu, T. Matsuishi, T. Iwamoto, R. Handa, K. Yoshioka, H. Kato, H. Ueda, S. Hara, H. Tabira, T. and Mekada, E.: "Elevated cerebrosspinal fluid levels of anti-CD9 antibodies in patients with subacute sclerosing panencephalitis."J. Infect. Dis.. 185. 1346-1350 (2002)
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[Publications] Iwamoto, R. Yamazaki, S. Asakura, M. Takashima, S. Hasuwa, H. Miyado, K. Adachi, S. Kitakaze, M. Hashimoto, K. Raab, G. Nanba, D. Higashiyama, S. Hori, M. Klagsbrun, M. and Mekada, E.: "HB-EGF and ErbB signaling is essential for heart function"Proc. Natl. Acad. Sci. U. S. A.. 100. 3221-3226 (2003)
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[Publications] Takeda, Y. Tachibana, I. Miyado, K. Kobayashi, M. Miyazaki, T. Funakoshi, T. Kimura, H. Yamane, H. Saito, Y. Goto, H. Yoneda, T. Yoshida, M. Kumagai, T. Osaki, T. Hayashi, S. Kawase, I. And Mekada, E..: "Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear phagocytes."J. Cell Biol.. In press.
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「研究成果報告書概要(欧文)」より
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