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2003 Fiscal Year Final Research Report Summary

Mechanisms of neuronal localization in the developing brain

Research Project

Project/Area Number 13480259
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKeio University (2002-2003)
Jikei University School of Medicine (2001)

Principal Investigator

NAKAJIMA Kazunori  Keio University, School of Medicine, Professor, 医学部, 教授 (90280734)

Co-Investigator(Kenkyū-buntansha) AJIOKA Itsuki  Keio University, School of Medicine, Instructor, 医学部, 助手 (10348790)
TABATA Hidenori  Keio University, School of Medicine, Instructor, 医学部, 助手 (80301761)
Project Period (FY) 2001 – 2003
Keywordscerebral cortex / projection neuron / interneuron / layer formation / reaggregation culture / ventricular zone / Reelin
Research Abstract

Cerebral cortical neurons form a six-layered structure in which their position depends on their birth-date. This developmental process requires the presence of Reelin, which is secreted by Cajal-Retzius cells in the cortical marginal zone (MZ). However, it is still unclear whether the migration from the ventricular zone (VZ) to beneath the MZ is essential for the projection neurons to segregate into layers. Previous transplantation studies of ferret cerebral cortical neurons suggested that their ultimate laminar fate is, at least to some extent, determined in the VZ, but it is unknown how "laminar fate" eventually positions cell in a specific layer. To explore the segregation properties of cortical cells that have not yet arrived beneath the MZ, embryonic day (E) 16 VZ and intermediate zone (IMZ) cells were dissociated and allowed to reaggregate for 1-4 days in vitro. The result suggested that the migrating neurons in the IMZ at E16 preferentially located near the center of the aggrega … More tes more than the proliferative cells in the VZ. The birth-date-labeling followed by the dissociation/reaggregation culture suggested that the segregation properties of the E16 IMZ was characteristic of the E14-born cells, which were migrating in the IMZ at E16, but they were not general properties of migrating IMZ cells. This birth-date-dependent segregation mechanism was also observed in the Reelin-signaling-deficient yotari cells. These findings suggest that cortical neurons acquire a birth-date-dependent segregation mechanism before their somas reach the MZ. In contrast to the projection neurons described above, gamma-aminobutyric acid (GABA)ergic interneurons arise in the ventral telencephalon and migrate into the cortical plate tangentially. Although the "inside-out" alignment of projection neurons in the cortex has been thoroughly analyzed, the pattern of interneuron alignment is not well understood. In this study, we show that in the postnatal day (P) 9.5 mouse visual cortex, GABAergic neurons born on embryonic day (E) 12.5 were distributed around two peak locations, mainly around layer V and also around layer II/III, while non-GABAergic neurons born on E12.5 were distributed around only one peak in layer VI. Both cell populations born on E15.5 exhibited only one common peak distribution in layer II/III. The two peak locations of GABAergic neurons born on E12.5 still existed at P30. When the subtypes of GABAergic neurons were analyzed, calretinin-positive cells born on E12.5 were distributed in the cortex around one peak location near layer II/III, whereas somatostatin-positive E12.5 cells were distributed in the cortex around one peak location near layer V. These results suggest that the alignment of interneurons is regulated differently according to subtypes and from that of projection neurons having the same embryonic day of origin. Less

  • Research Products

    (43 results)

All 2005 2004 2003 2002 2001 Other

All Journal Article (36 results) Book (6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Neuronal migration in cortical development2005

    • Author(s)
      Shigeaki Kanatani, Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      J.Child Neurol. 20(4)

      Pages: 274-279

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression profiles of EphA3 at both the RNA and protein level in he developing mammalian forebrain2005

    • Author(s)
      Chikako Kudo, Itsuki Ajioka, Yukio Hirata, Kazunori Nakajima
    • Journal Title

      J.Comp.Neurol. 487(3)

      Pages: 255-269

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Neuronal migration in cortical development.2005

    • Author(s)
      Shigeaki Kanatani, Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      J.Child Neurol. 20

      Pages: 274-279

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression profiles of EphA3 at both the RNA and protein level in the developing mammalian forebrain2005

    • Author(s)
      Chikako Kudo, Itsuki Ajioka, Yukio Hirata, Kazunori Nakajima
    • Journal Title

      J.Comp.Neurol. 487

      Pages: 255-269

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Components of the Reelin signaling pathway are expressed in the spinalcord.2004

    • Author(s)
      Yee Ping Yip, Christine Capriotti, Susan Magdaleno, David Benhayon, Tom Curran, Kazunori Nakajima, Joseph W. Yip.
    • Journal Title

      J.Comp.Neurol. 470(2)

      Pages: 210-219

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The Wnt/β-catenin pathway directs neuronal differentiation of cortical neural precursor cells2004

    • Author(s)
      Yusuke Hirabayashi, Yasuhiro Itoh, Hidenori Tabata, Kazunori Nakajima, Tetsu Akiyama, Norihisa Masuyama, Yukiko Gotoh
    • Journal Title

      Development 131(2)

      Pages: 2791-2801

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Birth-date dependent alignment of GABAergic neurons occurs in a different pattern from that of non-GABAergic neurons in the developing mouse visual cortex2004

    • Author(s)
      Masato Yozu, Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      Neurosci.Res. 49(4)

      Pages: 395-403

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 神経発達とその障害:皮質構造形成の分子メカニズムと統合失調症2004

    • Author(s)
      久保健一郎, 仲嶋一範
    • Journal Title

      医学のあゆみ 208(3)

      Pages: 127-132

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] リーリン(Reelin)2004

    • Author(s)
      関根克敏, 仲嶋一範
    • Journal Title

      分子精神医学 4(2)

      Pages: 160-161

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 放射状グリアの細胞移動様式2004

    • Author(s)
      衛藤薫, 仲嶋一範
    • Journal Title

      Clinical Neuroscience 22(7)

      Pages: 864

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 神経発生のダイナミクス(神経発生概論)2004

    • Author(s)
      四津真人, 仲嶋一範
    • Journal Title

      Clinical Neuroscience 22(9)

      Pages: 1004-1008

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Components of the Reelin signaling pathway are expressed in the spinal cord.2004

    • Author(s)
      Yee Ping Yip, Christine Capriotti, Susan Magdaleno, David Benhayon, Tom Curran, Kazunori Nakajima, Joseph W.Yip.
    • Journal Title

      J.Comp.Neurol. 470

      Pages: 210-219

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The Wnt/β-catenin pathway directs neuronal differentiation of cortical neural precursor cells.2004

    • Author(s)
      Yusuke Hirabayashi, Yasuhiro Itoh, Hidenori Tabata, Kazunori Nakajima, Tetsu Akiyama, Norihisa Masuyama, Yukiko Gotoh
    • Journal Title

      Development 131

      Pages: 2791-2801

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Birth-date dependent alignment of GABAergic neurons occurs in a different pattern from that of non-GABAergic neurons in the developing mouse visual cortex.2004

    • Author(s)
      Masato Yozu, Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      Neurosci.Res. 49(4)

      Pages: 395-403

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cell and molecular mechanisms that control cortical layer formation in the brain2003

    • Author(s)
      Ken-ichiro, Kubo, Kazunori Nakajima
    • Journal Title

      Keio J.Med. 52(1)

      Pages: 8-20

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cellular and molecular mechanisms of neuronal migration in neocortical development2003

    • Author(s)
      Takao Honda, Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      Sem.in Cell & Develop.Biol. 14(3)

      Pages: 169-174

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Segregation and coactivation of developing neocortical layer 1 neurons2003

    • Author(s)
      Takeshi Soda, Ryo Nakashima, Dai Watanabe, Kazunori Nakajima, Ira Pastan, Shigetada Nakanishi
    • Journal Title

      J.Neurosci. 23(15)

      Pages: 6272-6279

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Multipolar migration : the third mode of radial neuronal migration in the developing cerebral cortex2003

    • Author(s)
      Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      J.Neurosci. 23(31)

      Pages: 9996-10001

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 組織幹細胞:神経発生過程における細胞移動2003

    • Author(s)
      田畑秀典, 仲嶋一範
    • Journal Title

      再生医学(Molecular Medicine臨時増刊号) 40

      Pages: 287-297

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 「知っておきたい運動器の細胞」:ニューロン(神経細胞)2003

    • Author(s)
      仲嶋一範
    • Journal Title

      臨床整形外科 38(10)

      Pages: 1308-1310

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cell and molecular mechanisms that control cortical layer formation in the brain2003

    • Author(s)
      Ken-ichiro Kubo, Kazunori Nakajima
    • Journal Title

      Keio J.Med. 52(1)

      Pages: 8-20

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Segregation and coactivation of developing neocortical layer 1 neurons2003

    • Author(s)
      Takeshi Soda, Ryo Nakashima, Dai Watanabe, Kazunori Nakajima, Ira Pastan, Shigetada Nakanishi
    • Journal Title

      J.Neurosci. 23

      Pages: 6272-6279

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Multipolar migration : the third mode of radial neuronal migration in the developing cerebral cortex2003

    • Author(s)
      Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      J.Neurosci. 23

      Pages: 9996-10001

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Secreted Reelin molecules form homodimers2002

    • Author(s)
      Ken-ichiro Kubo, Katsuhiko Mikos hiba, Kazunori Nakajima
    • Journal Title

      Neurosci.Res. 43

      Pages: 381-388

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Neurons tend to stop migration and differentiate along the cortical internal plexiform zones in the Reelin signal-deficient mice2002

    • Author(s)
      Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      J.Neurosci.Res. 69

      Pages: 723-730

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 大脳皮質における神経細胞の誕生・移動と層形成機構2002

    • Author(s)
      味岡逸樹, 仲嶋一範
    • Journal Title

      医学のあゆみ 201(5)

      Pages: 295-301

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 大脳皮質形成のメカニズム2002

    • Author(s)
      仲嶋一範
    • Journal Title

      慶應医学 79(5,6)

      Pages: 135-143

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 神経細胞の移動と配置決定のメカニズム2002

    • Author(s)
      工藤千加子, 仲嶋一範
    • Journal Title

      Brain Medical 14(4)

      Pages: 371-377

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 大脳皮質神経細胞の移動様式2002

    • Author(s)
      田畑秀典, 仲嶋一範
    • Journal Title

      神経細胞移動と軸索伸長のダイナミクス(蛋白質核酸酵素) 47(15)

      Pages: 1991-2001

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Secreted Reelin molecules form homodimers2002

    • Author(s)
      Ken-ichiro Kubo, Katsuhiko Mikoshiba, Kazunori Nakajima
    • Journal Title

      Neurosci.Res. 43

      Pages: 381-388

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Efficient in utero gene transfer system to the developing mouse brains using electroporation -- Visualization of neuronal migration in the developing cortex2001

    • Author(s)
      Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      Neuroscience 103

      Pages: 865-872

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 大脳皮質層構造が形成されるメカニズム2001

    • Author(s)
      仲嶋一範
    • Journal Title

      神経化学 40

      Pages: 12-25

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 子宮内マウス胎児脳に対する電気穿孔法を用いた遺伝子導入法と神経細胞移動の可視化2001

    • Author(s)
      田畑秀典, 仲嶋一範
    • Journal Title

      実験医学 19

      Pages: 1251-1255

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Efficient in utero gene transfer system to the developing mouse brains using electroporation--Visualization of neuronal migration in the developing cortex2001

    • Author(s)
      Hidenori Tabata, Kazunori Nakajima
    • Journal Title

      Neuroscience 103

      Pages: 865-872

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Birthdate-dependent-segregation of the mouse cerebral cortical neuron in reaggregation cultures

    • Author(s)
      Itsuki Ajioka, Kazunori Nakajima
    • Journal Title

      Eur.J.Neurosci. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Birthdate-dependent-segregation of the mouse cerebral cortical neurons in reaggregation cultures

    • Author(s)
      Itsuki Ajioka, Kazunori Nakajima
    • Journal Title

      Eur.J.Neurosci. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 必ず上手くいく遺伝子導入と発現解析プロトコール(仲嶋一範, 北村義浩編)2003

    • Author(s)
      工藤千加子, 仲嶋一範
    • Total Pages
      180-192
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 必ず上手くいく遺伝子導入と発現解析プロトコール(仲嶋一範, 北村義浩編)2003

    • Author(s)
      田畑秀典, 仲嶋一範
    • Total Pages
      38-45
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Annual Review神経20032003

    • Author(s)
      仲嶋一範
    • Total Pages
      10-17
    • Publisher
      中外医学社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 日本生化学会バイオサイエンスの新世紀 第11巻 脳の発生・分化・可塑性(御子柴克彦, 清水孝雄編)2002

    • Author(s)
      仲嶋一範, 御子柴克彦
    • Total Pages
      67-76
    • Publisher
      共立出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 実験医学別冊 クローズアップ実験法総集編2002

    • Author(s)
      田畑秀典, 仲嶋一範
    • Total Pages
      162-166
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 脳神経外科学大系第一巻「神経科学」

    • Author(s)
      仲嶋一範, 本田岳夫, 久保健一郎
    • Publisher
      中山書店(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 中枢神経組織の再生方法2002

    • Inventor(s)
      仲嶋 一範, 味岡 逸樹
    • Industrial Property Rights Holder
      科学技術振興事業団
    • Industrial Property Number
      特願2002-335249号
    • Filing Date
      2002-11-18
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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