2002 Fiscal Year Final Research Report Summary
Development of method to make oocyte from male germ cells
| Project/Area Number |
13480279
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Gunma University |
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Principal Investigator |
HATADA Izuho Gene Research Center, Associate Proffessor, 遺伝子実験施設, 助教授 (50212147)
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| Co-Investigator(Kenkyū-buntansha) |
OBATA Yayoi Gene Research Center, Assistant Processor, 遺伝子実験施設, 助手 (70312907)
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| Project Period (FY) |
2001 – 2002
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| Keywords | imprinting / methylation / nuclear transplantation / germ cell / oocyte |
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| Research Abstract |
Genomicimprinitng refers to the differential expression of the two alleles of a gene, depending on their parental origins. In mammals, parental imprinting ensures the functional inequality of paternal and maternal genomes in the fertilized egg, and causes developmental failure of embryos produced by parthenogenesis or androgenesis. To elucidate the mechanisms of genomic imprinting, we examined whether maternal imprinit can be introduced to paternal genome in vitro.
First of all, we established in vitro system for completing maternal impriniting. Ovaries from mouse fetuses at 12.5 days post coitum were cultured for 17 days, followed by isolation of secondary follicles and further 11 days culture. During this period, maternal imprinitng was established in vitro.
Next we examined whether maternal imprinting can be introduced to the genome dermived from erased male germ cells by our system. Nuclear transplantation of nucleus from erased male germ cells into growing oocytes follwed by 10 days culture results in methylation of an imprinted gene Igf2r. This suggests that maternal imprint can be introduced to the paternal genome in vitro.
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Research Products
(18 results)
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[Publications] Higashimoto K, Soejima H, Yatsuki H, Joh K, Uchiyama M, Obata Y, Ono R, Wang Y, Xin Z, Zhu X, Masuko S, Ishino F, Hatada I, Jinno Y, Iwasaka T, Katsuki T, Mukai T: "Characterization and Imprinting Status of OBPH1/Obph1 Gene : Implications for an Extended Imprinting Domain in Human and Mouse"Genomics. 80・6. 575-584 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yatsuki H, Joh K, Higashimoto K, Soejima H, Arai Y, Wang Y, Hatada I, Obata Y, Morisaki H, Zhang Z, Nakagawachi T, Satoh Y, Mukai T: "Domain Regulation of Imprinting Cluster in Kip2/Lit1 Subdomain on Mouse Chromosome 7F4/F5 : Large-Scale DNA Methylation Analysis Reveals That DMR-Lit1 Is a Putative Imprinting Control Region"Genome Res. 12・12. 1860-1870 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Higashimoto K, Soejima H, Yatsuki H, Joh K, Uchiyama M, ObataY, Ono R, Wang Y, Xin Z, Zhu X. Masuko S, Ishino F, Hatada I, Jinno Y, Iwasaka T, Katsuki T, Mukai T.: "Characterization and Imprinting Status of OBPH1/Obph1 Gene : Implications for an Extended Imprinting Domain in Human and Mouse"Genomics. 80・6. 575-584 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yatsuki H, Job K, Higashimoto K, Soejima H, Arai Y, Wang Y, Hatada I, Obata Y, Morisaki H, Zhang Z, Nakagawachi T, Satoh Y, Mukai T.: "Domain Regulation of Imprinting Cluster in Kip2/Lit1 Subdomain on Mouse Chromosome 7F4/F5 : Large-Scale DNA Methylation Analysis Reveals That DMR-Lit1 is a Putative Imprinting Control Region"Genome Res.. 12・12. 1860-1870 (2002)
Description
「研究成果報告書概要(欧文)」より
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