2001 Fiscal Year Annual Research Report

日本産魚類のミトコンドリアゲノム・データベースの構築

Research Project

Project/Area Number	13556028
Research Institution	The University of Tokyo
Principal Investigator	西田睦東京大学, 海洋研究所, 教授 (90136896)
Co-Investigator(Kenkyū-buntansha)	宮正樹千葉県立中央博物館, 動物学研究科, 上席研究員 (30250137) 松浦啓一国立科学博物館, 動物研究部, 動物第二研究室室長 (70141984)
Keywords	魚類 / ミトコンドリアゲノム / mtDNA / 塩基配列 / データベース
Research Abstract	魚類は,資源としてさらに水圏生態系の構成要素として重要な生物グループである。その研究の深化には,卵や稚仔魚を含むあらゆるタイプの試料の種の正確な識別と分類,遺伝的多様性の把握,種内集団(系群)の峻別,分子系統分析などには,DNA塩基配列などの精緻な遺伝的情報が重要かつ不可欠になりつっある。しかし,DNA情報は急速に増大しており,その情報の流通の便を良くすることが強く求められている。そこで本研究では,上記のような目的にふさわしい情報源であることが明らかになっているミトコンドリアゲノムを取り上げ,有用性の高い「魚類のミトコンドリアゲノム・データベース」を構築することを目指す。この目標達成のために,今年度は,1)種々の魚類標本の入手を図り,国内および国外から新たに約250種の標本試料を入手した。2)われわれが開発したロングPCRと魚類ユニバーサルプライマーを活用する方法を用いて,新たに約50種のミトコンドリアゲノムの全塩基配列を決定した。また,約100種について,ミトコンドリアゲノムの部分配列を得た。3)得たDNAデータの検証可能性を維持するために,用いたDNA試料と魚体標本の国立科学博物館ないし千葉県中央博物館への登録を進めた。4)試料標本のデータベースおよびDNA塩基配列データベースの基本デザインを検討した。次年度には,さらに標本とDNAデータの収集を図るとともに,試料標本のデータベースおよびDNA塩基配列データベースのデザインをさらに改良し,それらの試用を始める予定である。

Research Products
(12 results)

All Other

All Publications (12 results)

[Publications] Inoue, Jun G.: "Complete mitochondrial DNA sequence of the Japanese anchovy, Engraulis japonicus"Fisheries Science. 67. 828-835 (2001)
[Publications] Aoyama, Jun: "Molecular phylogeny and evolution of the freshwater eel, genus Anguilla"Molecular Phylogenetics and Evolution. 20. 450-459 (2001)
[Publications] Ishikawa, Satoshi: "Genetic variation in the mitochondrial and nuclear DNA of the Jaanese conger Conger myriaster"Fisheries Science. 67. 1081-1087 (2001)
[Publications] Miya, Masaki: "Mitogenomic exploration of higher teleostean phylogenies: a case study for moderate-scale evolutionary genomics with 38 newly determined complete mitochondrial DNA sequences"Molecular Biology and Evolution. 18. 1993-2009 (2001)
[Publications] Inoue. Jun G: "A mitogenomic perspective on the basal teleostean phylogeny : resolving higher-level relationships with longer DNA sequences"Molecular Phylogenetics and Evolution. 20. 275-285 (2001)
[Publications] Kawaguchi, Akira: "Complete mitochondrial DNA sequence of Aulopus japonicus (Teleostei: Aulopiformes), a basal Eurypterygii : Longer DNA sequences and higher-level relationships"Ichthyological Research. 48. 213-223 (2001)
[Publications] Ishiguro, Naoya: "Complete mitochondrial DNA sequence of ayu, Plecoglossus altivelis"Fisheries Science. 67. 474-481 (2001)
[Publications] Inoue.Jun G: "Complete mitochondrial DNA sequence of Conger myriaster : Novel gene order for vertebrate mitochondrial genomes and the phylogenetic implications for anguilliform families"Journal of Molecular Evolution. 52. 311-320 (2001)
[Publications] Ishikawa, Satoshi: "Population structure of the Japanese eel Anguilla japonica as examined by mitochondrlal DNA seuencing"Fisheries Science. 67. 246-253 (2001)
[Publications] Suzuki, Nobuaki: "The phylogenetic position of the genus Atheresthes (Pleuronectidae) and its classification : A molecular phylogenetic approach using mitochondrial sequence data"Bulletin of Faculty of Fisheries Hokkaido University. 52. 39-46 (2001)
[Publications] Inoue, Jun G: "Complete mitochondrial DNA sequence of Japanese eel, Anguilla japonica"Fisheries Science. 67. 118-125 (2001)
[Publications] Lce, Jac-Seong: "The complete DNA scquence of the mitochondrial genome of the self-fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the first description of duplication of a control region in fish"Gene. 280. 1-7 (2001)

2001 Fiscal Year Annual Research Report

日本産魚類のミトコンドリアゲノム・データベースの構築

Principal Investigator

西田 睦 東京大学, 海洋研究所, 教授 (90136896)

Research Products

[Publications] Inoue, Jun G.: "Complete mitochondrial DNA sequence of the Japanese anchovy, Engraulis japonicus"Fisheries Science. 67. 828-835 (2001)

[Publications] Aoyama, Jun: "Molecular phylogeny and evolution of the freshwater eel, genus Anguilla"Molecular Phylogenetics and Evolution. 20. 450-459 (2001)

[Publications] Ishikawa, Satoshi: "Genetic variation in the mitochondrial and nuclear DNA of the Jaanese conger Conger myriaster"Fisheries Science. 67. 1081-1087 (2001)

[Publications] Miya, Masaki: "Mitogenomic exploration of higher teleostean phylogenies: a case study for moderate-scale evolutionary genomics with 38 newly determined complete mitochondrial DNA sequences"Molecular Biology and Evolution. 18. 1993-2009 (2001)

[Publications] Inoue. Jun G: "A mitogenomic perspective on the basal teleostean phylogeny : resolving higher-level relationships with longer DNA sequences"Molecular Phylogenetics and Evolution. 20. 275-285 (2001)

[Publications] Kawaguchi, Akira: "Complete mitochondrial DNA sequence of Aulopus japonicus (Teleostei: Aulopiformes), a basal Eurypterygii : Longer DNA sequences and higher-level relationships"Ichthyological Research. 48. 213-223 (2001)

[Publications] Ishiguro, Naoya: "Complete mitochondrial DNA sequence of ayu, Plecoglossus altivelis"Fisheries Science. 67. 474-481 (2001)

[Publications] Inoue.Jun G: "Complete mitochondrial DNA sequence of Conger myriaster : Novel gene order for vertebrate mitochondrial genomes and the phylogenetic implications for anguilliform families"Journal of Molecular Evolution. 52. 311-320 (2001)

[Publications] Ishikawa, Satoshi: "Population structure of the Japanese eel Anguilla japonica as examined by mitochondrlal DNA seuencing"Fisheries Science. 67. 246-253 (2001)

[Publications] Suzuki, Nobuaki: "The phylogenetic position of the genus Atheresthes (Pleuronectidae) and its classification : A molecular phylogenetic approach using mitochondrial sequence data"Bulletin of Faculty of Fisheries Hokkaido University. 52. 39-46 (2001)

[Publications] Inoue, Jun G: "Complete mitochondrial DNA sequence of Japanese eel, Anguilla japonica"Fisheries Science. 67. 118-125 (2001)

[Publications] Lce, Jac-Seong: "The complete DNA scquence of the mitochondrial genome of the self-fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the first description of duplication of a control region in fish"Gene. 280. 1-7 (2001)

西田睦東京大学, 海洋研究所, 教授 (90136896)