2002 Fiscal Year Final Research Report Summary
Analysis of mitochondrial DNA control region and forensic applications
| Project/Area Number |
13557036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Legal medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
FUKUSHIMA Hirofumi Shinshu Univ., Sch. Med., Professor, 医学部, 教授 (70135218)
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| Co-Investigator(Kenkyū-buntansha) |
ASAMURA Hideki Shinshu Univ., Sch. Med., assistant, 医学部, 助手 (80324250)
TAKAYANAGI Kayoko Shinshu Univ., Sch. Med., assistant, 医学部, 助手 (60313847)
OTA Msao Shinshu Univ., Sch. Med., lecturer, 医学部, 講師 (50115333)
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| Project Period (FY) |
2001 – 2002
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| Keywords | PCR / mitochondria / D loop / polymorphism / HV1 / HV2 / sequence / control region |
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| Research Abstract |
The small size of the mitochondrial DNA and its presence in many copies per cell make it useful for human DNA tyring when only small amounts of material are available or when the material is badly degraded. The sequence information of the mtDNA hypervariable regions 1 and 2 (HV1 and HV2) provided a robust tool for identification purposes relevant for the forensic science.
The mtDNA sequence were generated for 95 unrelated donors living in Japan. A total 91 different haplotypes were observed for HV1 and HV2 regions.
We typed six mutation sites in the coding region by use of amplified produet - length polymorphism (APLP) analysis. Two of these haplotypes, B1 and C2 were distributed in Japanese population. Moreover, the haplotypes A1, A2, B2, B3, and C2, were mostly restricted to the Mongoloid populations.
Personal identification were performed in 8 autopsy cases. Each cadaver was severely damaged, and neither fingerprint nor dental information could be obtained for personal identification. In all cases, it was possible to amplify and sequence the control regions, resulting in positive identification in 6 of 8 cases.
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