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2003 Fiscal Year Final Research Report Summary

Application of ghrelin for diagnosis and treatment of endocrine and metabolic diseases.

Research Project

Project/Area Number 13557090
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Endocrinology
Research InstitutionKurume University

Principal Investigator

KOJIMA Masayasu  Kurume University, Institute of Life Science, Professor, 分子生命科学研究所, 教授 (20202062)

Co-Investigator(Kenkyū-buntansha) MINAMITAKE Yoshiharu  Daiichi-Suntroy Pharma, Principal Investigator, 主任研究員
HAYASHI Yujiro  Daiichi-Suntroy Pharma, Principal Investigator, 主任研究員
KANGAWA Kenji  National Cardiovascular Center, Research Institute, Head, 生化学部, 部長(研究職) (00112417)
SAKATA Tuneaki  Shionogi Pharmapheutical, Research Institute, Head, 主任研究員
Project Period (FY) 2001 – 2003
Keywordsghrelin / growth hormone / appetite regulation / stomach / acyl acid / octanoic acid
Research Abstract

Our group discovered human and rat ghrelins from stomach. Ghrelin is a novel hormone, which stimulates growth hormone (GH) release and appetite. The third amino-acid serine from the N-terminal end is modified by a lipid n-octanoic acid and this modification is essential for ghrelin activity. In the period of this grant, we performed following research on ghrelin.
1, Prader-Willi syndrome (PWS) and ghrelin Using ghrelin ELISA system, we measured plasma ghrelin concentration in Prader-Willi patients with feeding disorder. We found high ghrelin concentration in PWS patients. We next examined the relationship between the age and the time when hyperphagia began, and found that plasma ghrelin concentration was already high after birth.
2, Creation of ghrelin null-mice We tried to create ghrelin null-mice and succeeded in creating ghrelin null-mice. We are now investigating their phenotypes.
3, Molecular forms of ghrelin in human stomach In human stomach ghrelin existed mainly as the n-octanoyl modified form. In addition, we found four minor molecular forms of ghrelin. Thus, in human stomach ghrelin existed as multiple forms with various amino-acid lengths and acyl-modifications.
4, Purifications and structures of non-mammalian ghrelins We determined the structures of amphibian, bird, and fish ghrelins, and found that ghrelin existed not only in mammals but also in all vertebrate species. Moreover, acyl-modification of ghrelin is found in amphibian, bird, and fish ghrelins, as like mammalian ghrelins. Thus, ghrelin is a well-conserved peptide hormone that is essential for survival of vertebrates.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] H.Kaiya et al.: "Peptide purification, complementary deoxyribonucleic acid (DNA) and genomicDNA cloning, and functinal characterizattion of ghrelin in rainbow trout"Endocrinology. 144. 5215-5226 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kaiya et al.: "Amidated fish ghrelin : purification, cDNA cloning in the Japanese eel and its biological activity"Journal of Endocrinology. 176. 415-423 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Choi et al.: "The role of ghrelin and growth hormone secretagogues receptor on rat adipogenesis"Endocrinology. 144. 754-759 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Hosoda et al.: "Structurural divergence of human ghrelin. Identification of multiple ghrelin-derived molecules produced by post-translational processing"Journal of Biological Chemistry. 278. 64-70 (2003)

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      「研究成果報告書概要(和文)」より
  • [Publications] K.Nakahara et al.: "Effect of chronic treatments with ghrelin on milk secretion in lactating rats"Biochem.Biophys.Res.Commun.. 303. 751-755 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tanaka et al.: "Ghrelin is involve in the decidualization of human endometrial stromal cells"J Clin Endocrinol Metab. 88. 2335-2340 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Choi K, Roh SG, Hong YH, Shrestha YB, Hishikawa D, Chen C, Kojima M, Kangawa K, Sasaki S.: "The role of ghrelin and growth hormone secretagogues receptor on rat adipogenesis."Endocrinology. 144(3). 754-759 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosoda H, Kojima M, Mizushima T, Shimizu 5, Kangawa K.: "Structural divergence of human ghrelin. Identification of multiple ghrelin-derived molecules produced by post-translational processing."J Biol Chem. 278(1). 64-70 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaiya H, Kojima M, Hosoda H, Moriyama S, Takahashi A, Kawauchi H, Kangawa K.: "Peptide purification, complementary deoxyribonucleic acid (DNA) and genomic DNA cloning, and functional characterization of ghrelin in rainbow trout."Endocrinology. 144(12). 5215-5226 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaiya H, Kojima M, Hosoda H, Riley LG, Hirano T, Grau EG, Kangawa K.: "Amidated fish ghrelin : purification, cDNA cloning in the Japanese eel and its biological activity."J Endocrinol. 176(3). 415-423 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakahara K, Hayashida T, Nakazato M, Kojima M, Hosoda H, Kangawa K, Murakami N.: "Effect of chronic treatments with ghrelin on milk secretion in lactating rats."Biochem Biophys Res Commun. 303(3). 751-755 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka K, Minoura H, Isobe T, Yonaha H, Kawato H, Wang DF, Yoshida T, Kojima M, Kangawa K, Toyoda N.: "Ghrelin is involved in the decidualization of human endometrial stromal cells."J Clin Endocrinol Metab. 88(5). 2335-2340 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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