2004 Fiscal Year Final Research Report Summary
Development of cancer modulation therapy with alternation of glycolchain associated with drug resistance
| Project/Area Number |
13557095
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
General surgery
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| Research Institution | Gunma University |
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Principal Investigator |
ASAO Takayuki Gunma University, Graduate School of Medicine, General Surgical Science, Associate Professor, 大学院・医学研究科, 助教授 (40212469)
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| Co-Investigator(Kenkyū-buntansha) |
KUWANO Hiroyuki Gunma University, Graduate School of Medicine, General Surgical Science, Professor, 大学院・医学研究科, 教授 (90186560)
SHIMURA Tatsuo Gunma University, Graduate School of Medicine, General Surgical Science, 医学部, 助手 (00282393)
YAZAWA Shin Instructor Japan Immunoresearch Laboratories Company Limited, vise president, 副所長
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| Project Period (FY) |
2001 – 2004
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| Keywords | 5FU / Metastasis / glycosvl-transferase / resistance / modulation therapy / リポソーム / アポトーシス |
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| Research Abstract |
Result I : In the present investigation, we found that the expression of these antigens bearing an alpha1,2-linked fucose correlated with the resistance of the tumor cells to anticancer treatments. Addition of an exogenous sugar acceptor for alpha1,2fucosyltransferase to the cell medium resulted in suppression of alpha1,2fucosylated antigen expression on the tumor cells and increased susceptibility to anticancer treatment. The increased susceptibility may be attributed to cancer cell-mediated priming by sugar acceptors for alpha1,2fucosyltransferase added to the medium.
Result II : Mouse colon cancer cells (colon26) were transfected with α(1→2)fucosyltransferase (FUT1). The glycosylation by FUT1 on the transfected cells was confirmed by flowcytometlic analysis. These cells were characterized by altered morphology and anchorage-independent growth. The sensitivity against hyperthermia by the transfection of α-1,2-fucosyltransferase to the mouse cancer cell line in vitro was increased. These findings could lead to a new approach toward the enhancement of heat sensitivity by a specific induction of glycosyl conjugates associated with α-1,2-fucosyltransferase.
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[Journal Article] alpha1-acid glycoprotein fucosylation as a marker of carcinoma progression and prognosis.2004
Author(s)
Hashimoto S, Asao T, Takahashi J, Yagihashi Y, Nishimura T, Saniabadi AR, Poland DC, van Dijk W, Kuwano H, Kochibe N, Yazawa S.
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Journal Title
Cancer 101(12)
Pages: 2825-2836
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Tumor-related expression of alpha1,2fucosylated antigens on colorectal carcinomacells and its suppression by cell-mediated priming using sugar acceptors for alpha1,2fucosyltransferase.2002
Author(s)
Yazawa S, Nishimura T, Ide M, Asao T, Okamura A, Tanaka S, Takai I, Yagihashi Y, Saniabadi AR, Kochibe N.
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Journal Title
Glycobiology 12(9)
Pages: 545-553
Description
「研究成果報告書概要(欧文)」より
