2003 Fiscal Year Final Research Report Summary
Gene transfer technique for clinical islet transplantation
| Project/Area Number |
13557096
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
General surgery
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| Research Institution | Sakura National Hospital (2002-2003)
Chiba University (2001) |
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Principal Investigator |
KENMOCHI Takashi Sakura National Hospital, Institute of Clinical Research, Researcher, 臨床研究部, 研究員 (50215133)
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| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Michihiro Sakura National Hospital, Institute of Clinical Research, Researcher, 臨床研究部, 研究員
SAIGO Kenichi Sakura National Hospital, Institute of Clinical Research, Researcher, 臨床研究部, 研究員 (60323424)
SAKAMOTO Kaoru Sakura National Hospital, Institute of Clinical Research, Researcher, 臨床研究部, 室長 (30360689)
山田 研一 国立佐倉病院, 臨床研究部, 部長
ASANO Takehide Chiba Cancer Center, Department of Gastoenteral surgery, Head
WATANABE Satomi Sakura National Hospital, Institute of Clinical Research, Researcher
AKATSU Naotake University Hospital, Department of Surgery II, Medical Stuff
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| Project Period (FY) |
2001 – 2003
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| Keywords | islet transplantation / islet isolation / Gene transfer / Clinical islet transplantation / Lipofection |
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| Research Abstract |
Study 1.Gene transfer into rat islets. Islets were isolated from F344 rat and cultured for 3-7 days before gene transfer. Gene transfection was made using polyamine transfection reagents. Vector was pZeoSV2/lacZ. Gene transferred islets survived for 5 days with good endocrine function. Gene transfer rate evaluated by X-gal staining showed >90%. Study 2.Gene transfer into canine islets. PCMS-EGFP was transferred into the islats from Beagle pancreata using 1)Gene gun, 2)Electroporarion, and 3)Lipofection. Lipofection technique was simple and less harmful for the isles. Moreover, insulin release was well preserved with Lipofection technique. Study 3.Clinical islet isolation, cryopreservation, and transplantation. Eight human islet isolations were performed in our institute using one brain-dead donor and seven non-heart beating donors. Islet yield was from 18693 to 491040 IEq. Purity was from 30 to 80 %. One of these isolations were used for clinical islet allograft. After transplantation, hypoglycemia disappered and obtained stable control of blood sugar levels.
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