2002 Fiscal Year Final Research Report Summary
Detection of sleep regulating genes by DNA microarray to apply for clinical anesthesia
| Project/Area Number |
13557128
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Hirosaki University |
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Principal Investigator |
TAKAHASHI Satoshi Hirosaki University, University Hospital, Senior Instructor, 医学部附属病院, 助手 (10236276)
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Ken-ichi Hirosaki University, School of Medicine, Associate Professor, 医学部, 助教授 (20165468)
SAKAI Tetsuhiro Hirosaki University, University Hospital, Associate Professor, 医学部附属病院, 助教授 (80205707)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Anesthesia / sleep / DNA / Microarray / RNA / gene |
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| Research Abstract |
Sleep and anesthesia have common characteristics, e.g. loss of consciousness. In deed, some hypnotic agents like benzodiazepines were used in the area of clinical anesthesia. Physiological sleep also has important functions including protection of brain or enhancement of immune system. Recently, many endogenous substances regulating sleep (sleep factors) were studied. Particularly, interleukin-1, growth hormone releasing hormone and prostaglandin D2 are very notable. However, it is still unknown what sleep factor is the most important. Furthermore, relationship between sleep and anesthesia is not studied so well. Finally, there is almost no study to apply the endogenous sleep factors for clinical anesthesia. In the present study, we detected main sleep related genes by DNA microarray. We found that expressions of some genes related to endogenous sleep factors, e.g. interleukin-1 and growth hormone releasing hormone, were enhances after 8-h sleep deprivation in the rat whole brain. Then, we found that sleep deprivation make the anesthetic potency strong in rats. In conclusion, some sleep factors or their related substances may be used as anesthetic supplements.
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