2002 Fiscal Year Final Research Report Summary
ヒト顎関節円板・滑膜病変に対するANK遺伝子を用いた遺伝子診断法の開発
| Project/Area Number |
13557175
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MURAKAMI Kenichiro KYOTO UNIVERSITY, Graduate School of Medicine, Associete. Prof., 医学研究科, 助教授 (00174269)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Akira KYOTO UNIVERSITY, Graduate School of Medicine, Prof., 医学研究科, 教授 (00162694)
YASUDA Shinya KYOTO UNIVERSITY, Graduate School of Medicine, Assistant Prof., 医学研究科, 助手 (50263075)
KATSU Takashi KYOTO UNIVERSITY, Graduate School of Medicine, Assistant Prof., 医学研究科, 助手 (90314202)
SUGAI Manabu KYOTO UNIVERSITY, Center for Moleculor Biology & Genetics, Assistant Prof., 遺伝子実験施設, 助手 (90303891)
YAMAMURA Isao KYOTO UNIVERSITY, Graduate School of Medicine, Assistant Prof., 医学研究科, 助手 (90332733)
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| Project Period (FY) |
2001 – 2002
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| Keywords | temporomandibular joint / gene diagnosis / ANK gene / synovial cell / ortiomlar disk |
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| Research Abstract |
1, Human homologue of ANK gene (ANKH) expression in. synovial membrane and articular disk of human temporonmandibuiar joint (TMJ)
We had established the primary culture system of human synovial cells erived from articuluar disks which were surgically dissected out on temporomandibular disorder (T.MMD) patients. Total RNA was. extracted from 5 different kind of these cells. RT-PCR as performed by using,specific primers for ANKH. Specific band was found in all cases. It was confirmed that this band represented ANKH by direct sequencing. Therefore, we confirmed ANKH mRMA expression in synovial membrane of human TMJ.
2, Mutation analysis, of ANKH gene in internal derangement of TMJ
Human gene analysis -in TMD patients was approved by the medical ethics committee of Kyoto University on December 9, 2001 (#G86). After informed consent was obtained, we took 10ml of blood from patients which had TMJ internal. derangement and closed lock. 80 samples were collected and stored so far. Genomic DNA was extracted. In 12 cases, promoter region and every 11 exons were amplified and purified. Then they were directly sequenced in coding region and exon-introm boundary. We found 3 novel polymorphism in exon 2, 8 and intron 10. Furthermore, we identified multiplication of GGC triple repeat in Promoter region. As to GGC triple repeat, we are working on case-control study using many-cases.
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Research Products
(21 results)
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[Journal Article] Histone accetylation determines the developmentally regulated accessibility for T cell receptor-gene recombination.2001
Author(s)
Agata, Y., Katakai, T., Ye, S.-K., Sugai, M., Gonda, H., Honjo, T., Ikuta, K., Shimizu, A.
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Journal Title
J.Exp.Med. 193
Pages: 873-879
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Targeting of Kuruppel-associated box-containing zinc-finger proteins to centromeric heterochromatin : implication for the gene silencing mechanisms.2001
Author(s)
Matsuda, E., Agata, Y., Sugai, M., Katakai, T., Gonda, H., Shimizu, A.
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Journal Title
J.Biol.Chem. 267
Pages: 14222-14229
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] DACH : Genomic characterization, evaluation as a candidate for postaxial polydactyly type A2, and developmental expression pattern of the murine homologue.2001
Author(s)
Ayres, J., Shum, L., Akarsu, N., Dashner, R., Takahashi, K., Ikura, T., Slavkin, Nuckolls, G.H.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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