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2003 Fiscal Year Final Research Report Summary

Genetic diagnosis and immunoglobulin A receptor targeting therapy for periodontitis

Research Project

Project/Area Number 13557189
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Periodontal dentistry
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

KOBAYASHI Tetsuo  NIIGATA UNIVERSITY, Medical and Dental Hospital, Lecturer, 医歯学総合病院, 講師 (00215344)

Co-Investigator(Kenkyū-buntansha) SUGITA Noriko  NIIGATA UNIVERSITY, Graduate School of Medical and Dental Sciences, Assistant, 大学院・医歯学総合研究科, 助手 (30313547)
Project Period (FY) 2001 – 2003
KeywordsPeriodontitis / Polymorphism / Immunoglobulin / FcαR / Bispecific antibody / Porphyromonas gingivalis / Neutrophils / Targeting therapy
Research Abstract

-1.Genetic diagnosis of periodontisis risk :
Sequence analyses of immunoglobulin A and G Fc receptor (neutrophil-specific FcαRI, CD89 and B cell-specific FcγRIIB, CD32) indicated one and seven single-nucleotide substitutions, respectively. Of these polymorphisms, FcαRI nt 324A/G and FcγRIIB nt 775T/C have been shown to be associated with aggressive periodontitis risk. Neutrophils (PMN) from peripheral blood and gingival crevicular fluid (GCF) of periodontitis patients exhibited a decreased phagocytosis in the nt 324 A/A as compared to the nt 324 G/G patients. These results suggest FcαRI nt 324A/A patients to be high-susceptibility to aggressive periodontitis.
-2.Immunoglobulin A receptor targeting therapy :
The bispecific antibody (BsAb) was constructed from anti-Porphyromonas gingivalis and anti-FcαRI monoclonal antibody (MAb). Flow cytometric analyses showed opsonic activity for GCF PMN to be comparable between BsAb and control anti-P. gingivalis MAb. However, a BsAb significantly promoted phagocytosis and killing activity of GCF PMN, which exhibiting higher FcαRI expression levels than PB PMN. These data suggest BsAb targeting toward GCF PMN FcαRI to be effective for improvement of antibacterial immunity in periodontitis patients.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Tetsuo Kobayashi et al.: "Risk of Periodontitis in Systemic Lupus Erythematosus is Associated with Fcγ Receptor Polymorphisms"Journal of Periodontology. Vol.74. 378-384 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiko Yasuda et al.: "FcγRIIB Gene Polymorphisms in Japanese Periodontitis Patients"Genes and Immunity. Vol.4. 541-546 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kouji Yamamoto et al.: "Association of Fcγ receptor IIa genotype with chronic periodontitis in Caucasians"Journal of Periodontology. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Susumu Kaneko et al.: "A novel polymorphism of FcαRI (CD89) associated with aggressive periodontitis"Tissue Antigens. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuko Shimada et al.: "Association of tumor necrosis factor receptor type 2 +587 gene polymorphism with severe chronic periodontitis"Journal of Clinical Periodontology. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gonzales JR et al.: "Interleukin-4 gene polymorphisms in Japanese and Caucasian patients with aggressive periodontitis"Journal of Clinical Periodontology. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kobayashi, S.Ito, K.Yamamoto, H.Hasegawa, N.Sugita, T.Kuroda, S.Kaneko, I.Narita, K.Yasuda, M.Nakano, F.Gejyo, H.Yoshie.: "Risk of Periodontitis in Systemic Lupus Erythematosus Is Associated with Fcγ Receptor Polymorphisms.."Journal of Periodontology. Vol.74, No.3. 378-384 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yasuda, N.Sugita, K.T.Kobayashi, Yamamoto, H.Yoshie: "Seven single nucleotide substitutions in human Fcγ receptor IIB gene."Genes and Immunity. Vol.4, No.8. 541-546 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamamoto, T.Kobayashi, S.Grossi, AW.Ho, R.J.Genco, H.Yoshie, E.De Nardin.: "Association of Fcγ receptor IIa genotype with chronic periodontitis in Caucasians"Journal of Periodontology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kaneko, T.Kobayashi, K.Yamamoto, M.D.Jansen, J.G.J.van de Winkel, H.Yoshie.: "Anovel polymorphism of FcαRI (CD89) associated with aggressive periodontitis."Tissue Antigens. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y, Shimada, H.Tai, M.Endo, T.Kobayashi, K.Akazawa, K.Yamazaki, H.Yoshie: "Association of tumor necrosis factor receptor type 2 +587 gene polymorphism with severe chronic periodicities"Journal of Clinical Periodontology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] JR.Gonzales, T.Kobayashi, J.Michel, M.Mann, H.Yoshie, J.Meyle: "Interleukin-4 gene polymorphisms in Japanese and Caucasian patients with aggressive periodontitis"Journal of Clinical Periodontology. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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