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2004 Fiscal Year Final Research Report Summary

Construction of Environmental Biremediation Enzymes Whose Catalysis is Regulated by Light and Molecular Switches

Research Project

Project/Area Number 13558069
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 環境保全
Research InstitutionTohoku University

Principal Investigator

SHIMIZU Toru  Tohoku University, Institute of Multidisciplinary Research for Advanced Materials, Research Associate, 多元物質科学研究所, 教授 (40118956)

Co-Investigator(Kenkyū-buntansha) KUROKAWA Hirofumi  Tohoku University, Institute of Multidisciplinary Research for Advanced Materials, Research Associate, 多元物質科学研究所, 助手 (80359546)
IGARASHI Jotaro  Tohoku University, Institute of Multidisciplinary Research for Advanced Materials, Research Associate, 多元物質科学研究所, 助手 (80375162)
Project Period (FY) 2001 – 2004
KeywordsMolecular switch / Sensor enzyme / Heme sensor / cAMP / Protein microarray / Protein-protein interaction / redox states / Signal transduction
Research Abstract

Cytochrome P450s activate carcinogenetic activities and toxicities of various environmental chemicals including benzpyrene, aflatoxin, haloethanes. However, cytochrome P450s are also involved in metabolism and degradation of various environmental chemicals. Therefore, it is possible that we can construct efficient and useful environmental bioremediation enzymes if the degradation properties of cytochrome P450s can be used. A physiologically important gas, nitric oxide (NO), is synthesized by nitric oxide synthase whose active site structure is the sameas those of cytochrome P450s. On the other hand, there are many sensor enzymes and sensor proteins, where electron introduction, light illumination, bindings of calcium ion, CO, NO, molecule oxygen and various carcinogens induced certain protein conformational changes. The changes became signals that transmit to catalytic domain and switch the catalysis on/off. In the present study, we examined electron transfer process and protein-protein interactions of a calcium sensor protein, neuronal NO synthase. Then, we constructed chimeric enzymes composed of the calcium sensor domain of NO synthase and the have-bound active site of cytochrome P450. We examined efficiencies of electron transfer and catalysis of the generated calcium sensor chimeric enzymes with the an of a novel molecular switching enzyme. It was found that the chimeric enzyme functions as a calcium sensor enzyme. Furthermore, we examined structure and function relationships of a new redox sensor enzyme, Ec DOS. It was found that Ec DOS is also CO and NO sensor enzyme, because the catalysis was strongly inhibited by CO and NO. As the last step of this project study, we characterized potential heme-sensor enzyme, SOUL. In summary, the study provided the possibility to construct novel sensor enzymes with efficient biodegradation properties of environmental chemicals.

  • Research Products

    (8 results)

All 2004 2002 2001

All Journal Article (8 results)

  • [Journal Article] SOUL in Mouse Eyes Is a New Hexameric Heme-binding Protein2004

    • Author(s)
      E.Sato
    • Journal Title

      Biochemistry 43(44)

      Pages: 14189-14198

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Catalytically Functional Flavocytochrome Chimera of P450 and NO synthase2002

    • Author(s)
      S.Fujiwara
    • Journal Title

      Journal of Inorganic Biochemistry 91(4)

      Pages: 515-526

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization of a Direct Oxygen Sensor Heme Protein from E.coli2002

    • Author(s)
      Y.Sasakura
    • Journal Title

      Journal of Biologicsl Chemistry 277(26)

      Pages: 23821-23827

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Catalytically Functional Flavocytochrome Chimera of P450 and NO Synthase2002

    • Author(s)
      S.Fujiwara
    • Journal Title

      Journal of Inorganic Biochemistry 91(4)

      Pages: 515-526

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of a Direct Oxygen Sensor Heme Protein from E.coli2002

    • Author(s)
      Y.Sasakura
    • Journal Title

      Journal of Biological Chemistry 277(26)

      Pages: 23821-23827

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Control of Electron Transfer in Neuronal NO Synthase2001

    • Author(s)
      S.Daff
    • Journal Title

      Biochemical Society Transactions 29(2)

      Pages: 147-152

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intra-subunit and Inter-subunit Electron Transfer in Neuronal NO Synthase2001

    • Author(s)
      I.Sagami
    • Journal Title

      Journal of Biological Chemistry 276(32)

      Pages: 30036-30042

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Rapid Calmodulin-dependent Interdomain Electron Transfer in NO Synthase2001

    • Author(s)
      K.Kobayashi
    • Journal Title

      Journal of Biological Chemistry 276(43)

      Pages: 39864-39871

    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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