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2003 Fiscal Year Final Research Report Summary

Practical use of cloning technology for NOD and dy disease model mice production

Research Project

Project/Area Number 13558099
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory animal science
Research InstitutionTOKYO UNIVERSITY OF AGRICULTURE

Principal Investigator

KONO Tomohiro  TOKYO UNIVERSITY OF AGRICULTURE, DEPARTMENT OF BIOSCIENCE, PROFESSOR, 応用生物科学部, 教授 (80153485)

Co-Investigator(Kenkyū-buntansha) ITO Mamoru  CENTRAL INSTITUTE FOR EXPERIMENTAL ANIMALS, IMMUNOLOGY LABORATORY, CHIEF RESEARCHER, 免疫研究室, 室長 (00176364)
NAGAFUCHI Seiko  KYUSHU UNIVERSITY, FACULTY OF MEDICINE AND SCHOOL OF HEALTH SCIENCES, PROFESSOR, 医療技術短期大学部, 教授 (00150441)
Project Period (FY) 2001 – 2003
KeywordsNOD mice / dy mice / mdx mice / embryo cloning / ES cells / disease model mice
Research Abstract

The present study was conducted to develop embryo cloning systems using ES cell and contribute to fundamental studies for disease and its treatments. Here, we focused on NOD non-obese diabetic mouse used as a model for diabetes typeI, and dy and mdx mice used as muscular dystrophy and tried to produce cloned mice from ES cells derived from above disease model mice. The results obtained were summarized as follows. 1)By standard nuclear transfer, cloned blastocysts were successfully produced using ES cells established from NOD mice, but no pup was obtained to date. 2)ES cells, which can be cultured over several passages, have been established from dy and mdx mice. 3)Using these ES cells as donor nuclei, cloned blastocysts were produced, but again no pup was obtained. Together, we have established ES cells from NOD, dy and mdx mice, but failed to produce cloned individuals. Further studies may be necessary to clarify developmental competence of ES cells established here.

  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Ono Y, Shimozawa N, Muauruma K, Kimoto S, Hioki K, Ito M, Kono T.: "Production of cloned mice from embryonic stem cells arrested at metaphase"Reproduction. 122. 731-736 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sotomaru Y, Katsuzawa Y, Domeki I, Kono T.: "Determination by real-time RT-PCR of imprinted expression of the insulin-like growth factor II(Igf2)gene in mouse uninarental fetuses"J.Reprod.Dev.. 47. 139-144 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ono Y, Shimozawa N, Ito M, Kono T.: "Cloned mice from fetal fibroblast cells arrested at metaohase by a serial nuclear transfer"Biol.Reprod.. 64. 44-50 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagafuchi S.: "AIRE gene expression in peripheral monocytes and dendritic cells and its possible role in antigen presentation"Keio J.Med.. 50. 118-119 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河野友宏: "ES細胞を用いたクローンマウス"医学のあゆみ. 203. 1061-1064 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimozawa N.Ono Y.Kimoto S, Hioki K, Araki Y, Kono T, Ito M.: "Abnormalities in cloned mice are not transmitted to the progeny"Genesis. 34. 203-207 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河野友宏: "体細胞クローン技術と再生医療"整形・災害外科. 45. 1040-1041 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimozawa N, Ono Y, Muguruma K, Hioki K, Araki Y, Kono T, Ito M.: "Direct production of gene-targeted mice from ES cells by nuclear transfer and gene transmission to their progeny"Exp.Anim.. 51. 375-381 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sotomaru Y.Katsuzawa Y.Hatada I, Obata Y, Sasaki H, Kono T.: "Unrequited expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses"J.Biol.Chem.. 277. 12474-12478 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kono T, Sotomaru Y, L Dandolo.: "Mouse parthenogenetic embryos with monoallelic H19 expression can develop to day 17.5 of gestation"Dev.Biol.. 243. 294-300 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kogawa K.Nagafuchi S, Katsuta H, Kudoh J, Shimizu N, Harada M.: "Expression of AIRE gene in peripheral monocyte/dendritic cell lineage"Immunol.Lett.. 80. 195-198 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kogawa K, Kudoh J.Nagafuchi S.Ohga S, Hara T, Shimizu N.: "Distinct clinical phenotype and immunoreactivitv in Jananese siblings with autoimmune polyglandular syndrome type 1(APS-1) associated with compound heterozygous hovel AIRE gene mutations"Clin.Immunol.. 103. 277-283 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimoda K, Kamesaki K, Numata A, Nagafuchi S, Harada M.: "Tyk is required for the induction and nuclear translocation of Daxx which regulates IFN-a-induced suppression of B lymphocyte formation"J.Immunol.. 169. 4707-4711 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagafuchi S.: "Epstein-Barr virus survival : expression and release of Fas ligand."Intern.Med.. 41. 603-604 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimozawa N, Tajima S, Azuma N, Hioki K, Kono T, Ito M.: "Histological study of the hypertrophic placentas and open eyelids observed in cloned fetuses"J.Reprod.Dev.. 49. 221-226 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 塩田邦郎, 松林秀彦, 河野友宏 他(共著): "永井書店"妊娠の生物学. 396 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木秋悦, 佐藤英明, 河野友宏 他(共著): "養賢堂"卵子研究法. 405 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ono Y, Shimozawa N, Muguruma K, Kimoto S, Hioki K, Tachibana M, Shinkai Y, Ito M, Kono T.: "Production of cloned mice from embryonic stem cells arrested at metaphase."Reproduction. 122. 731-736 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sotomaru Y, Katsuzawa Y, Domeki I, Kono T.: "Determination by real-time RT-PCR of imprinted expression of the insulin-like growth factor II(Igf2)gene in mouse uniparental fetuses."J.Reprod.Dev.. 47. 139-144 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ono Y, Shimozawa N, Ito M, Kono T.: "Cloned mice from fetal fibroblast cells arrested at metaphase by a serial nuclear transfer."Biol.Reprod.. 64. 44-50 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagafuchi S.: "AIRE gene expression in peripheral monocytes and dendritic cells and its possible role in antigen presentation."Keio J Med. 50. 118-119 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kono T.: "ES cloned mice."Progress in Medicine. 203. 1061-1064 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimozawa N, Ono Y, Kimoto S, Hioki K, Araki Y, Shinkai Y, Kono T, Ito M.: "Abnormalities in cloned mice are not transmitted to the progeny."Genesis. 34. 203-207 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kono T.: "Somqatic cloning and regenerative medicine."Orthopedics -disaster surgery. 45. 1040-1041 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimozawa N, Ono Y, Muguruma K, Hioki K, Araki Y, Shinkai Y, Kono T, Ito M.: "Direct production of gene-targeted mice from ES cells by nuclear transfer and gene transmission to their progeny."Exp.Anim.51. 51. 375-381 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sotomaru Y, Katsuzawa Y, Hatada I, Obata Y, Sasaki H, Kono T.: "Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses."J.Biol.Chem.. 277. 12474-12478 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kono T, Sotomaru Y, L Dandolo.: "Mouse parthenogenetic embryos with monoallelic H19 expression can develop to day 17.5 of gestation."Dev.Biol.. 243. 294-300 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kogawa K, Nagafuchi S, Katsuta H, Kudoh J, Tamiya S, Sakai Y, Shimizu N, Harada M: "Expression of AIRE gene in peripheral monocyte/dendritic cell lineage."Immunol Lett. 80. 195-198 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kogawa K, Kudoh J, Nagafuchi S, Ohga S, Katsuta H, Ishibashi H, Harada M, Hara T, Shimizu N.: "Distinct clinical phenotype and immunoreactivity in Japanese siblings with autoimmune polyglandular syndrome type 1 (APS-1) associated with compound heterozygous novel AIRE gene mutations."Clin Immunol. 103. 277-283 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimoda K, Kamesaki K, Numata A, Aoki K, Matsuda T, Oritani K, Tamiya S, Kato K, Takase K, Imamura R, Yamamoto T, Miyamoto T, Nagafuji K, Gondo H, Nagafuchi S, Nakayama K, Harada M.: "Cutting edge: tyk2 is required for the induction and nuclear translocation of Daxx which regulates IFN-alpha-induced suppression of B lymphocyte formation."J Immunol. 169. 4707-4711 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagafuchi S.: "Epstein-Barr virus survival : expression and release of Fas ligand."Intern.Med.(Editorial). 41. 603-604 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimozawa N, Tajima S, Azuma N, Hioki K, Kono T, Ito M.: "Histological study of the hypertrophic placentas and open eyelids observed in cloned fetuses."J.Reprod.Dev.. 49. 221-226 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kono T.: "Biology of Pregnancy"Nagai press. 359-365 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kono T.: "Study in oocytes."Yokendo Ltd. 17-23 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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