2002 Fiscal Year Final Research Report Summary
Development of UV excitation CARS microscopy system and applications to biological tissue measurement
| Project/Area Number |
13558112
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Osaka University |
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Principal Investigator |
HASHIMOTO Mamoru Osaka University, Graduate School of engineering science, Associate Professor, 大学院・基礎工学研究科, 助教授 (70237949)
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| Co-Investigator(Kenkyū-buntansha) |
CHIKAMORI Kensuke Natuto University of education, Department of Basic Human Science for Integrated Studies, Associate Professor, 学校教育学部, 助教授 (40108874)
YASUI Takeshi Osaka University, Graduate School of engineering science, Research Associate, 大学院・基礎工学研究科, 助手 (70314408)
ARAKI Tsutomu Osaka University, Graduate School of engineering science, Professor, 大学院・基礎工学研究科, 教授 (50136214)
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| Project Period (FY) |
2001 – 2002
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| Keywords | multi-photon microscopy / Raman Spectroscopy / CARS / 3D imaging / Molecular Structure / Nonlinear Optics / Biomedical measurements |
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| Research Abstract |
We developed a multi-focus CARS microscopy system using two synchronized ps-Ti : Sapphire lasers. CARS microscope require two lasers with different wavelength, high peek intensity, narrow spectral bandwidth around a few cm^<-1>, and large wavelength scanning range about 2000cm^<-1>. Therefore, we developed a system using two synchronized ps-Ti : Sapphire lasers for light source. A micro-lens array is used to excite and observe parallel points for shortening the measurement time. The second harmonics of lasers are also available to use for electric resonance enhancement.
For the polystyrene beads without absorption at near-IR and UV, the CARS spectrum and image are obtained by near-IR excitation only, because of low intensities of UV beam. On the other hand for human erythrocyte which has absorption around 400 nm, Clear CARS spectrum and image are obtained by UV excitation, because of electric resonance enhancement.
As the results, it was confirmed that the electric resonance enhancement is the important for CARS microscopy.
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