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2003 Fiscal Year Final Research Report Summary

The preparation of global distribution map of Paramecium and its endonuclear symbiotic bacterium Holospora

Research Project

Project/Area Number 13575007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field 生態
Research InstitutionYamaguchi University

Principal Investigator

FUJISHIMA Masahiro  Yamaguchi University, Faculty of science, Professor, 理学部, 教授 (40127783)

Co-Investigator(Kenkyū-buntansha) DOHRA Hideo  Shizuoka University, Institute far f genetic Research and Biotechnology, Assistant, 遺伝子実験施設, 助手 (10311705)
Project Period (FY) 2001 – 2003
KeywordsHolospora / paramecium / Geographic distribution / Endonuclear symbiotic bacterium / Stress resistance / Cell suraface proteins
Research Abstract

(1) A new Parameciuim species was found from water samples collected from Shanghai, China, and the species was named as Paramecium schewiakoffi (in press)
(2) For the first time, we collected P. decaurelia and P. dodecaurelia in Japan (Pryzybos et al., Folia boil., 51, 223-224, 2003)
(3) A new Holospora species was found in the macronucleus of P.bursaria collected in Morioka city, and the bacterium was named as Holospora hiwatashii (in preparation)
(4) N.obtusa bearing P.caudatum was collected in Yamaguchi city. This is the most Southern place where Holospora species had ever collected
(5) Collection records on Holospora species by H.-D.Gortz, S.I.Fokin and M.Fujishima for the past 20 years were collected and edited
(6) We found that infection of Holospora transforms the host cells to various sress-resistant, and clarified molecular mechanism of this phenomenon (Hori and Fujishima, J.Euk. Microbiol., 50, 293-298, 2003 ; Nakamura et al., in preparation)
(7) We found 20 host genes, of which expressions were altered by infection of H.obtusa by the differential display method, and four genes were cloned (Nakamura et al., in preparation ; Aikawa et al, in preparation)

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] I.N.Skovorodkin, S.I.Fokin, M.Fujishima.: "Fates of the endonuclear symbiotic bacteria Holospora obtusa and Holospora undulata injected into the macronucleus of Paramecium caudatum"European Journal of Protistology. 237. 137-145 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Sugii, M.Fujishima: "Purification of GVBD-inducing protein from the ciliate Tetrahymena thermophila."Journal of Eukaryotic Microbiology. 48. 414-424 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Hori, M.Fujishima: "Influence of the endosymbiotic bacterium Holospora obtusa against heat-shock response of the host Paramecium caudatum"Journal of Eukaryotic Microbiology. 50. 293-298 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.I.Fokin, M.Schweikert, H.-D.Gortz, M.Fujishima: "Bacterial endocytobionts of Ciliophora. Diversity and some interactions with the host."European Journal of Protistology. 39. 475-480 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E.Przybos, M.Fujishima, Y.Nakaoka.: "Paramecium decaurelia and Paramecium dodecaurelia from the P.aurelia spp. complex in Japan."Folia biologica. 51. 223-224 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.I.Fokin, E.Przybos, S.M.Chivilev, C.L.Beier, M.Horn, B.Skotarczak, B.Wodecka, M.Fujishima: "Morphological and molecular investigations of Paramecium schewiakoffi sp.nov.(Ciliophora, Oligohymenophorea) and current status of paramecia distribution and taxonomy."European Journal of Protistology. 40(In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤島政博: "生物学入門(大学生のための基礎シリーズ2)(石川統編)(川口明彦, 藤島政博, 大森正之, 石川 統, 西駕秀俊, 塚谷裕一, 上村慎治, 和田 勝, 嶋田正和 著)"(株)東京化学同人. 283 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I.N.Skovorodkin, S.I.Fokia, M.Fujishima: "Fates of the endonuclear symbiotic bacteria Holospora obtusa and Holospora undulata injected into the macronucleus of Paramecium caudatum"European Journal of Protistology. 237. 137-145 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Sugii, M.Fujishima: "Purification of GVBD-inducing protein from t he ciliate Tetrahymena thermophila"Journal of, Eukaryotic Microbiology. 46. 414-424 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Hori, M.Fujishima: "Influence of the endosymbiotic bacterium Holospora obtusa against heat-shack response of the host Paramecium caudatum"Journal of Eukaryotic Microbiology. 50. 293-298 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.I.Fokin, M.Schweikert, H.-D.Gonz, M.Fujishima: "Bacterial endocytobionts of Ciliophora. Diversity and some interactions with the host"European Journal of Protistology. 39. 475-480 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E.Przybos, M.Fujishima, Y.Nakaoka: "Paramecium decaurelia and Paramecium dodecaurelia from the P.aurelia spp. complex in Japan"Folia biologics. 51. 223-224 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.I.Fokin, E.Przybos, S.M.Chivilev, C.L.Beier, M.Horn, B.Skotarczak, B.Wodecka, M.Fujishima: "Morphological and molecular investigations of Paramecium schewiakoffi sp.nov. (Ciliophora, Oligohymenophorea) and current status of paramecia distribution and taxonomy"European Journal of Protistology. 40(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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