2002 Fiscal Year Final Research Report Summary
Survey for the cause of emerging dengue haemorrhagic fever in Southeast Asia
| Project/Area Number |
13576016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Virology
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| Research Institution | Nagasaki University |
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Principal Investigator |
MORITA Kouichi Nagasaki University, Institute of Tropical Medicine, Professor, 熱帯医学研究所, 教授 (40182240)
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| Co-Investigator(Kenkyū-buntansha) |
HASEBE Futoshi Nagasaki University, Institute of Tropical Medicine, Assistant Professor, 熱帯医学研究所, 講師 (20253693)
TSUDA Yoshio National Institute of Infectious Diseases, Chief, 昆虫医科学部, 室長 (20207393)
INOUE Shingo Nagasaki University, Institute of Tropical Medicine, Research Associate, 熱帯医学研究所, 助手 (00346925)
KUMATORI Atsushi Nagasaki University, Institute of Tropical Medicine, Assistant Professor, 熱帯医学研究所, 講師 (60244092)
TADANO Masayuki Ryukyu University, School of Medicine, Associate Professor, 医学部, 助教授 (80179712)
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| Project Period (FY) |
2001 – 2002
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| Keywords | dengue haemorrhagic fever / Southeast Asia / dengue virus / tropical diseases |
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| Research Abstract |
We conducted a study to find out the causes of dengue hemorrhagic fever (DHF) and its emergence in Southeast Asia, by : (a)isolating the dengue virus in many parts of the region ; (b)analyzing patient cells infected by the virus ; and (c)conducting a vector mosquito survey. We then analyzed the following three factors of the disease : 1)pathogen ; 2)host ; and 3)vector. In Thailand, a combination of dengue virus strains and the sensitivity of the mosquito vector was found to be associated with the spread of the virus. In the Philippines, a genotype shift of dengue virus type2 across the country was observed. In Vietnam, a new genotype of dengue type4 virus outbreak was witnessed. Also, Flowcytometry analysis in the Philippines and Vietnam demonstrated multipopulation of dengue virus infected cells in peripheral blood monocytes.
In the past, two hypothesis on DHF were advocated, i.e., 1)virus virulence theory and 2)secondary infection theory. Our conclusion from these series of research is that both factors were concerned with the development, emergence and expansion of DHF in Southeast Asia. The evident physiological characteristic of DHF is its rapid plasma leakage from peripheral vessels. It should be clarified what viral protein(s) and kind of host cytokines are stimulating such rapid leak of plasma. It has also been noted that each population of Aedes aegypti caught from different localities demonstrated a unique susceptibility to dengue virus strains. The genotype shift of dengue 2 virus observed in the Philippines may have been promoted by a higher affinity of the newly introduced genotype of the virus. This indicates the possibility that rapid expansion of DHF during the 1960s may have been promoted by any virus mutation which enhanced virus and vector mosquito affinity. Further genetic analysis of dengue virus strains are necessary to clarify the mechanism of DHF emergence and rapid expansion in Southeast Asia.
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Research Products
(24 results)
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[Journal Article] Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Assay.2005
Author(s)
Parida M, Horioke K, Ishida H, Dash PK, Saxena P, Mukul A, Jana, Islam M, Inoue S, Hosaka N, Morita K
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Journal Title
Journal of Clinical Microbiology 43
Pages: 2895-2903
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Comparison of Clinical Features and Hematologic Abnormalities Between Dengue Fever and Dengue Hemorrhagic Fever Among Children in the Philippines.2005
Author(s)
Celia C.Carlos, Kazunori Oishi, ^* Maria T.D.D.Cinco, Cynthia A.Mapua, Shingo Inoue, Deu John M.Cruz, Mary Ann M.Pancho, Carol Z.Tanig, Ronald R.Matias, Kouichi Morita, Filipinas F.Natividad, Akira Igarashi, Tsuyoshi Nagatake
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Journal Title
Am.J.Trop.Med.Hyg. Vol.73
Pages: 435-440
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fusion PCR generated Japanese encephalitis virus/dengue 4 virus chimera exhibits lack of neuroinvasiveness, attenuated neurovirulence, and a dual-flavi immune response in mice.2004
Author(s)
Mathenge EGM, ParquetMC, Funakoshi Y, Houhara S, Wong PF, Ichinose A, Hasebe F, Inoue S, Morita K
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Journal Title
Journal of General Virology. 85
Pages: 2503-2513
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Association of increased platelet-associated immunoglobulins with thrombocytopenia and the severity of disease in secondary dengue virus infections.2004
Author(s)
Saito M, Oishi K, Inoue S, Dimaano EM, Alera MT, Robles AM, Estrella BD Jr, Kumatori A, Moji K, Alonzo MT, Buerano CC, Matias RR, Morita K, Natividad FF, Nagatake T
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Journal Title
Clin Exp Immunol. Vol.138(2)
Pages: 299-303
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fusion PCR generated Japanese encephalitis virus/dengue 4 virus chimera exhibits lack of neuroinvasiveness, attenuated neurovirulence, and a dual-flavi immune response in mice.2004
Author(s)
Edward Gitau Matumbi Mathenge, Maria del Carmen Parquet, Yasutomo Funakoshi, Seiji Houhara, Pooi, Fong Wong, Akitoyo Ichinose, Futoshi Hasebe, Shingo Inoue, Kouichi Morita
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Journal Title
Journal of General Virology. Vol.85
Pages: 2503-2513
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Distribution of three arbovirus antibodies among monkeys (Macaca facscicularis) in the Philippines.2003
Author(s)
Inoue S, Morita K, Matias RR, Tuplano JV, Resuello RR., Candelario JR, Cruz DJM, Mapua CA, Hasebe F, Igarashi A, Natividad FF
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Journal Title
Journal of Medical Primatology. 32
Pages: 89-94
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Correlation between increased platelet-associated IgG and thrombocytopenia in secondary dengue virus infections.2003
Author(s)
Ohishi K., Inoue S., Cinco M., Diaano E., Alera M., Alfon J., Abanes F., Crus D., Matias R., Matsuura H., Hasebe F., Tanimura S., Kumatori A., Moirta K., Natividad F., Nagatake T.
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Journal Title
J.Med.Virol. 71
Pages: 259-264
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Distribution of three arbovirus antibodies among monkeys (Macaca fascicularis) in the Philippines.2003
Author(s)
Inoue S., Morita K., Matias R.R., Tuplano J.V., Resuello R.R.G., Candelario J.R., Cruz D.J.M., Mapua C.A., Hasebe F., Igarashi A., Natividad F.F.
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Journal Title
J.Med.Primatol. 32
Pages: 89-94
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Combined Detection and Genotyping of Chikungunya Virus by a Specific Reverse Transcription-Polymerase Chain Reaction.2002
Author(s)
Hasebe F., Parquet M.C., Pandey B.D, Mathenge E.G.M., Morita K., V.Balasubramaniam V., Saat Z., Yusop A., Sinniah, M., Natkunam S., Igarashi K.
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Journal Title
J.Med.Virol. 67
Pages: 370-374
Description
「研究成果報告書概要(欧文)」より
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