2002 Fiscal Year Final Research Report Summary
Identification and characterization of the meteor protein, a novel heterochromatin-related protein in Drosophila.
| Project/Area Number |
13640614
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
遺伝
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
H.INOUE Yoshihiro Drosophila Genetic Resource Center, Lecturer, ショウジョウバエ遺伝資源センター, 講師 (90201938)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Masamitsu Faculty of textile science, Department of applied biology, Professor, 繊維学部, 教授 (00182460)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Mitosis / Chromosome / heterochromatin / Drosophila |
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| Research Abstract |
A novel mitotic mutant, mtr^2, which had been isolated from our large-scale screens of Drosophila mitotic mutants, showed a high proportion of mitotic cells containing hyper-condensed chromatids that have undergone anaphase separation but that the segregation was prevented. Cytological examination of cells in the larval central nervous system from mtr^2 revealed that chromosome fragmentation occurred frequently at centromeres in the mutant cells. The mtr^2 cells contained abnormal mitotic spindles consisted of a less number of spindle microtubules. In the mtr^2 cells, chromatid separation can occur before metaphase even though they have the spindle defects. The abnormal anaphase-like cells in mtr^2 contained a comparable level of cyclin B to the normal level in metaphase cells. On the base or these cytological observations, we proposed a model that the primary defect or the mtr^2 is a perturbation of centromer structure and that it possibly leads to a loss of sister chromatid connection at centromere and further results in a failure of release from an active state of mitotic checkpoint after a completion of mitotic spindle formation. Genetic analyses of the mtr^2 revealed that the chromosome contains two separate mitotic mutations, a novel lethal mutation l(3)acc6487 and a female and male sterile mutation mtr^2. The mitotic phenotypes concerned with construction of centromeres corresponded with the l(3)acc6487 mutation. On the other hand, the mtr gene is required for a negative regulation of meiotic entry in Drosophila males. The mtr gene was localized to 98C region on the polytene chromosome and cloned the genomic region including this gene by a plasmid rescue. The mtr gene corresponds to the known gene, dlarp encoding a of the Lupas Antigen-related protein. A fine chromosome mapping for gene cloning of the l(3)acc6487 is now underway.
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[Publications] Higashiyama, H., Hirose, F., Yamaguchi, M., Inoue, Y.H., Fujikake, N., Matsukage, A., Kakizuka, A.: "Identification of ter94, Drosophila VCP, as a modulator of polyglutamine-induced eye degeneration"Cell Death Differ.. 9. 264-273 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kwon, E.-J., Oh, E.-J., Kim, Y.-S., Hirose, F., Ohno, K., Nishida, Y., Matsukage, A., Yamaguchi, M., Yoo, M.-A.: "Transcriptional regulation of the Drosophila raf proto-oncogene by the transcription factor dE2F"Nucleic Acids Res.. 29. 1808-1814 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamaguchi, M., Yoshida, H., Hirose, F., Inoue, Y. H., Hayashi, Y., Yamagishi, M., Nishi, Y., Tamai, K., Sakaguchi, K., Matsukage, A.: "Ectopic expression of BEAF32A in the Drosophila eye imaginal disc inhibits differentiation of photoreceptor cells and induces apoptosis"Chromosomal. 10. 313-321 (2001)
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[Publications] Yoshida, H., Inoue, Y. H., Hirose, F., Sakaguchi, K., Matsukage, A., Yamaguchi, M.: "Overexpression of DREF in the Drosophila wing imaginal disc induces apoptosis and a notching wing phenotype"Genes to Cells. 6. 877-886 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Hirose, F., Ohshima, N., Shiraki, M., Inoue, Y. H., Taguchi, O., Nishi, Y., Matsukage, A., Yamaguchi, M.: "Ectopic expression of DREF induces DNA synthesis, apoptosis and unusual morphogenesis in the Drosophila eye imaginal disc: possible interaction with Plycomb and trithorax group proteins"Mol. Cell. Biol.. 21. 7231-7242 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Takata, K., Yoshida, H., Hirose, F., Yamaguchi, M., Kai, M., Oshige, M., Sakimoto, I., Koiwai, O., Sakaguchi, K.: "Drosophila mitochondrial transcription factor A: Characterization of its cDNA and expression pattern during development"Biochem. Biophys. Res. Commun.. 287. 474-483 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Higashiyama, H., Hirose, F., Yamaguchi, M., Inoue, Y. H., Fujikake, N., Matsukage, A., Kakizuka, A.: "Identification of ter94, Drosophila VCP, as e modulator of polyglutamine-induced neurodegeneration"Cell Death and Differentiation. 9. 264-273 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hwang, M.-S., Kim, Y.-S., Choi, N.-H., Park, J.-H., Oh, E.-J., Kwon, E.-J., Yamaguchi, M., Yoo, M, -A.: "The Caudal homeodomain protein activates Drosophila E2F gene expression"Nucleic Acids Res.. 30. 5029-5035 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Kwon, E., Seto, H., Hirose, F., Ohshima, N., Takahashi, Y., Nishida, Y., Yamaguchi, M.: "Transcription control of a gene for Drosophila transcription factor, DREF by DRE and ciselements conserved between Drosophila melanogaster and virilis"Gene. in press.
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[Publications] Nagai, Y., Fujikake, N., Ohno, K., Higashiyama, H., Popiel, H. A., Rahadian, J., Yamaguchi. M., Strittmatter, W. J., Burke, J. R., Toda, T.: "Prevention of polyglutamine oligomerization and neurodegeneration by the peptide inhibitor QBP1 in Drosophila"Hum. Mol. Genet.. in press.
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「研究成果報告書概要(欧文)」より
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[Publications] Takata, K., Inoue, Y. H., Hirose, F., Murakami, S., Shimanouchi, S., Sakimoto, I., Sakaguchi, K.: "Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development"Cell Biology International. 27. 361-374 (2003)
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[Publications] Mathe, E., Inoue, Y. H., Palframan, W., Brown, G., Glover, D. M.: "Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions anddevelopment of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis"Development. 130. 901-915 (2003)
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「研究成果報告書概要(欧文)」より
