2003 Fiscal Year Final Research Report Summary
Establishment of monitoring system of the gene-expression both of the host legume and its microsymbiont.
| Project/Area Number |
13640653
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Kagoshima University |
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Principal Investigator |
ABE Mikiko Kagoshima University, Faculty of Science, Professor, 理学部, 教授 (00107856)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Akihiro Kagoshima University, Faculty of Science, Assistant Professor, 理学部, 助手 (50305108)
UCHIUMI Toshiki Kagoshima University, Faculty of Science, Associate Professor, 理学部, 助教授 (20193881)
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| Project Period (FY) |
2001 – 2003
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| Keywords | symbiotic hemoglobin / non-symbiotic hemoglobin / leghemoglobin / Lotus japonicus / promoter / transformation / hairly root / GFP |
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| Research Abstract |
Lotus japonicus is a promising model legume for studying molecular interactions in symbiotic nitrogen fixation and whole genome sequence of microsymbiont of Lotus japonicus, Mesorhizobium loti MAFF303099, is known. Transcriptional profiles of whole genome of M. loti revealed that the symbiosis island (611 kb region on the chromosome) functions as clustered expression islands to support symbiotic nitrogen fixation. A gene disruption of 1-aminocyclopropane-1-carboxylate deaminase, which is highly up-regulated in bacteroid (symbiotic state of rhizobia), decreased competitiveness on nodule formation. This suggests that disruption of highly expressed genes is a useful strategy for exploring novel gene functions in symbiosis.
A fusion gene of promoter region of a symbiotic hemoglobin gene (LjLb3) of L. japonicus and the structural gene of GFP was constructed and introduced into L. japonicus mediated by Agrobacterum tumefaciens or A. rhizogenes. Inoculation of M. loti to transgenic plants roots and hairy roots resulted in normal nodule formation. The expression of the fusion gene was confirmed by RT-PCR and GFP-expression could be observed by fluorescent microscopy during nodulation process. Interestingly, fluorescent cells were scattered on the surface of the roots after a few hours later of rhizobial inoculation. Using transgenic plants and transgenic hairy roots, quantitative observation of host plant cells that responding to rhizobia could be performed.
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[Publications] Suzuki, A., Akune, M., Kogiso, M., Imagama, Y., Osuki, K., Uchiumi, T., Higashi, S., Han, S.Y., Yoshida, S., Asami, T., Abe, M.: "Control of nodule number by the phytohormone abscisic acid in the roots of two leguminous species."Plant Cell Physiol.. 45(in press). (2004)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Uchiumi, T., Oowada, T., Itakura, M., Mitsui, H., Nukui, N., Dawadi, P., Kaneko, T., Tabata, S., Yokoyama, T., Tejima, K., Saeki, K., Oomori, H., Hayashi, M., Maekawa, T., Sriprang, R., Murooka, Y., Tajima, S., Simomura, K., Nomura, M., Suzuki, A., Shimoda, Y., Sioya, K., Abe, M., Minamisawa, K.: "Expression islands clustered on symbiosis island of Mesorhizobium loti genome."J.Bacteriol.. 45. 2439-2448 (2004)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ngom, A., Nakagawa, Y., Sawada, H., Tsukahara, J., Wakabayashi, S., Uchiumi, T., Nuntagij, A., Kotepong, S., Suzuki, A., Higashi, S., Abe, M.: "A novel symbiotic nitrogen-fixing member of Ochrobactrum isolated from root nodule of Acacia tree."J.Gen.Appl.Microbiol.. 50. 17-27 (2004)
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「研究成果報告書概要(欧文)」より
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[Publications] Tani, C., Sasakawa, H., Takenouchi, K., Abe, M., Uchiumi, T., Suzuki, A., Higashi, S.: "Isolation of endophytic Frankia from root nodules of Casuarina equisetifolia and infectivity to host plants."Soil Sci.Plant.Nutri.. 9. 137-142 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kaneko, T., Nakamura, Y., Sato, S., Minamisawa, K., Uchiumi, T., Sasamoto, S., Watanabe, A., Idesawa, K., Iriguchi, M., Kawashima, K., Kohara, M., Matsumoto, M., Shimpo, S., Tsuruoka, H., Wasa, T., Yamada, M., Tabata, S.: "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDAI 110."DNA Res.. 9. 189-197 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kaneko, T., Nakamura, Y., Sato, S., Minamisawa, K., Uchiumi, T., Sasamoto, S., Watanabe, A., Idesawa, K., Iriguchi, M., Kawashima, K., Kohara, M., Matsumoto, M., Shimpo, S., Tsuruoka, H., Wasa, T., Yamada, M., Tabata, S.: "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110 (Supplement)."DNA Res.. 9. 225-256 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Uchiumi, T., Shimoda, Y., Suzuki, A., Tsuruta, T., Mukoyoshi, Y., Suzuki, A., Senoo, K., Sato, S., Kato, T., Tabata, S., Higashi, S., Abe, M.: "Expression of symbiotic and nonsymbiotic globin genes responding to microsymbionts on Lotus japonicus."Plant Cell Physiol.. 43. 1351-1358 (2002)
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「研究成果報告書概要(欧文)」より
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