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2002 Fiscal Year Final Research Report Summary

Mechanism of morphological development of mycelia and its application on production of microbial product

Research Project

Project/Area Number 13650854
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionSHIZUOKA UNIVERSITY

Principal Investigator

PARK Enoch y.  Shizuoka University, Department of Applied Biological Chemistry, Professor, 農学部, 教授 (90238246)

Co-Investigator(Kenkyū-buntansha) OBAKE Mitsuyasu  Shizuoka University, Department of Applied Biological Chemistry, Professor, 農学部, 教授 (50224051)
Project Period (FY) 2001 – 2002
KeywordsImage analysis / Flow through chamber / Mycelial morphology / Arachidonic acid / Mortierella alpina / Fungus
Research Abstract

The intrastructure of mycelial pellets of Mortierella alpina and the morphological parameters, such as hyphal growth rate, tip formation rate, tip extension rate and branch formation rate of Mortierella alpina have been investigated in this research. The intrastructure of mycelial pellets were visualized following labeling with fluorescein isothiocyanate (FITC) and Nile red using fluorescence microscopy. The pellet was an ellipse shape, but its intrastructure was shaped as doughnut and a cave was present inside, which was clear by judging 3-D image of intrastructure of pellet. Using image analysis, the fatty acid production was shown on the verge of pellet, which was corresponded on the place of dense mycelia. The cavity ratio was calculated from area based on the fluorescent intensity and remained at 20% in a 50-L fermentor but finally increased to 35%. Mycelial pellet volume was paralleled to the cavity ratio.
Using a flow-through chamber under 25 different combinations of carbon and nitrogen concentrations, morphological parameters were measured and found that they were influenced not by C/N ratio but by carbon concentration in medium. Specific rates of hyphal growth and tip formation remained at constant at lower carbon concentration of 5 g/l, respectively. Tip extension rate from one tip was 60 (μm/tip/h) in a carbon concentration below 15 g/l, and branching formation rate was independent upon carbon concentration. Tip extension rate was a function of specific hyphal growth rate, and the specific hyphal growth rate was linearly proportional to the specific tip formation rate, which demonstrated that tip extension rate was exponentially proportional to the specific tip formation rate. Branch formation rate per hyphal element remained unchanged even at lower tip extension rate than 60 (μm/tip/h) and at lower specific hyphal growth rate than 0.83 h^<-1>, respectively.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Enoch Y.Park, T.Hamanaka, K.Higashikawa, S.Fujikawa: "Monitoring of morphological development of arachidonic acid producing filamentous microorganism Mortierella alpina"Appl. Microbiol. Biotechnol.. 59. 706-712 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Hamanaka, K.Higashikawa, S.Fujikawa, Enoch Y.Park: "Mycelial pellet intrastructure and visualization of mycelia and intracellular lipid in a culture of Mortierella alpina"Appl. Microbiol. Biotechnol.. 56. 233-238 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Koike, H.Cai, K.Higashikawa, S.Fujikawa, Enoch Y.Park: "Effect of consumed carbon to nitrogen source ratio on mycelial morphology and arachidonic acid productioin in culture of Mortierella alpina"J. Biosci. Bioeng.. 91. 382-389 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Enoch Y.Park, Y.Koike, K.Higashikawa, S.Fujikawa, M.Okabe: "Effect of nitrogen source on mycelial morphology and arachidonic acid in cultures of Mortierella alpina"J. Biosci. Bioeng.. 88. 61-67 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 朴 龍洙: "培養環境による糸状菌の菌糸形態の変化および画像解析による菌糸形態の解析"日本農芸化学会誌. 76. 947-950 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 朴 龍洙: "糸状菌ペレットの内部構造"バイオサイエンスとインダストリー. 59. 825-828 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 朴 龍洙: "バイオプロセスシステムエンジニアリング, 清水浩編集"シーエムシ出版. 251-265 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Enoch Y.Park, T.Hamanaka, K.Higashiyama, and S.Fujikawa: "Monitoring of morphological development of arachidonic acid producing filamentous microorganism Mortierella alpina"Appl.Microbiol.Biotechnol.. 59. 706-712 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Hamanaka, K.Higashiyama, S.Fujikawa, and Enoch Y.Park: "Mycelial pellet intrastructure and visualization of mycelia and intracellular lipid in a culture of Mortierella alpina"Appl.Microbiol.Biotechnol.. 56. 233-238 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Koike, H.J.Cai, K.Higashiyama, S.Fujikawa, and Enoch Y.Park: "Effect of consumed carbon to nitrogen source ratio on mycelial morphology and arachidonic acid production in culture of Motierella alpina"J.Biosci.Bioeng. 91(4). 382-389 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Enoch Y.Park, Y.Koike, K.Higashiyama, S.Fujikawa, and M.Okabe: "Effect of nitrogen source on mycelial morphology and arachidonic acid production in cultures of Mortierella alpina"J.of Biosci.Bioeng. 88(1). 61-67 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Enoch Y.Park: "Morphological change in fungal cultivation and application of image analysis"Nippon Nogeikagaku Kaishi. 76. 947-950 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Enoch Y.Park: "Intrastructure of mycelial pellet"Biosci.and Bioind. 59. 825-828 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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