2002 Fiscal Year Final Research Report Summary
Near-field Scanning Optical Microscopy and Nano-electrophoresis
| Project/Area Number |
13650867
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
工業分析化学
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
HORIE Kazuyuki Fac. Technology, Professor, 工学部, 教授 (10013690)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Satoshi The Queen's University of Belfast, Assistant, 数理学部, 助手 (60313003)
FURUKAWA Hidemitsu Fac. Technology, Assistant, 工学部, 助手 (50282827)
MIYASHITA Yoshiharu Fac. Technology, Assistant, 工学部, 助手 (00293259)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Nanooptics / Near-field scanning optical microscope / NSOM / Nanoelectrophoresis / Polystyrene gel / Latex / Refractive index control / Fluorescence control |
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| Research Abstract |
In this research we constructed a near-field scanning optical microscope (NSOM) which can be applied to study electrophoresis in nano space, and aimed at the separation analysis of fluorescent-labeled polymer particles and biomaterials and monitoring of material transfer on nanometer scale resolution. Fluorescent images of polystyrene latex moving in a specially-prepared liquid electrophoresis cell with 3 mm width, 25 mm length, and 150 μm depth were observed on the NSOM stage. Brownian motion of the polystyrene latex loosely interacting with the poly-N-isopropylacrylamide network was noticed. Fluorescent images of a single core-shell type microparticle as well as photochemically-induced refractive index and fluorescence patterning were also studied with this NSOM system.
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