2002 Fiscal Year Final Research Report Summary
A studv on the structure and function of a fungal β-1, 6-glucanase
| Project/Area Number |
13660077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Tohoku University |
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Principal Investigator |
NAKAJIMA Tasuku Tohoku University, Graduate School of Agricultural Science, Division of Life Science, Professor, 大学院・農学研究科, 教授 (20091720)
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| Project Period (FY) |
2001 – 2002
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| Keywords | β-glucanase / β-1,6-glucanase gene / Neurospora crassa / fungal cell wall gene / 1β-1,6-glucan / 1β-1,6-glucanase gene disruptant |
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| Research Abstract |
A gene (neg1) encoding endo-β-1,6-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-β-1,6-glucanpse protein of 463 amino acids and signal peptide of 17 amino.acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed β-1,6-D-glucanase activity. No homologous genes are found in yeasts. and fungi.
The β-1,6-glucanase gene (neg1) was disrupted by using repar-induced point mutation(RIP). The gene disruptant strain (R12-1) had no β-1,6-glucanase activity. The phenotype analyses showed thet the sisuptant had no apparent morphological changes, but protein denaturing agents, such as SDS or CTAB inhibited the mycerial growth.
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