2002 Fiscal Year Final Research Report Summary
Characterization of His-Asp phosphorelay system in S.pombe and E. coli.
| Project/Area Number |
13660087
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Nagoya University |
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Principal Investigator |
AIBE Hirofumi Nagoya University, Graduate School of Bioagricultural. Sciences, Associate Professor., 大学院・生命農学研究科, 助教授 (60211687)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Escherichia coli / fission yeast / signal transduction / micro array / His-Asp phosphorelay / stress response / His-Kinase / Response regulator |
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| Research Abstract |
1. Characterization of His-Asp phosphorelay system in fission yeast
I found that :
(1) Three histidine kinases (HK) found in that genome sequence of fission yeast are redundantly involved in the G2/M cell cycle control.
(2) The HKs work upstream of Spy1 and Mcs4, the HPt protein and response regulator, respectively.
(3) The Prrl, a response regulator, is involved in both meiosis and oxidative stress response. In this process, the HKs may be involved as oxidative stress sensors.
2. Characterization of His-Asp phosphorelay system in E. coli.
I found that :
(1) A sole HPt protein in E. coli, YojN, functions as a phosphate mediator between RcsC (HK) and RcsB (response regulator) that is involved in the capsule synthesis.
(2) There may be functional interactions (such as cross talk) among His-Asp phosphorelay systems based on the microarray analysis of all two-component regulatory system mutants of E. coli. In this analysis, I found the cellular functions, such as RpoS regulon and chemotaxis, are affected by multiple His-Asp phoshorelay systems.
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Research Products
(12 results)
