2002 Fiscal Year Final Research Report Summary
Physiological function of Na^+/K^+ transporters from bacteria and plants
| Project/Area Number |
13660088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | NAGOYA UNIVERSITY |
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Principal Investigator |
UOZUMI Nobuyuki Nagoya University, Bioscience Center Associate Professor, 生物分子応答研究センター, 助教授 (40223515)
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| Project Period (FY) |
2001 – 2002
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| Keywords | salt / ion transport system / Arabidopsis thaliana / HKTl / transporter / channel / K^+ / Na^+ |
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| Research Abstract |
1. AtHKTl from Arabidopsis (AtHKTl) was found to form 4-fold membrane-pore-membrane structure.
2. It is found that the P-loop of HKT transporters show identity only in one of glycine residues that mediates K^+ selectivity. HKT transporters share a common ancestor transporter with 4-loops.
3. KATl shares typical similarity with other animal and plant K^+ channels. The voltage-sensing segments of S3 and S4 in KATl are posttranslationally integrated into the membrane only when specific interation occurs between them. The insertion process is a newly recognized second type of the membrane protein integration process.
4. The aspartate in the transmembrane of Na^+/H^+ antiporter from a bacteria involves in H^+ permeation but not Na^+ permeation.
5. The AtHKTl was expressed in vascular tissue in plant.
6. AtHKTl plays a role in the recirculation of Na^+ in plant body.
7. The K^+ permeation of HKT-type transporter in Synechocystis PCC6803 was measured. K^+ transport activity was activated by Na^+ using E. coli expression system.
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Research Products
(17 results)
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[Publications] Kato,Y., Sakaguchi,M., Mori Y., Saito,K., Nakamura,T., Bakker,E.P., Sato,Y., Goshima,S. and Uozumi,N.: "Evidence in support of a four transmembrane-pore-transmembrane topology model for the Arabidopsis thaliana Na^+/K^+ translocating AtHKTl protein, a member of the superfamily of K^+ transporters"Proc.Natl.Acad.Sci.USA.. 98. 6488-6493 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakamura,T., Fujisaki,Y., Enomoto,H., Nakayama,Y., Takabe,T., Yamaguchi,N., and Uozumi,N.: "Residue aspartate-147 from the third transmembrane region of Na^+/H^+ antiporter NhaB of Vibrio aliginolyticus plays a role in its activity"J.Bacteriol.. 183. 5762-5767 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Sato,Y., Sakaguchi,M., Goshima,S., Nakamura,T., and Uozumi,N.: "Integration of Shaker-type K^+ channel, KATl into the endoplasmic reticulum membrane : Synergistic insertion of voltage sensing segments, S3-S4 and independent insertion of pore-forming segments, S5-P-S6"Proc.Natl.Acad.Sci.USA.. 99. 60-65 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Maser,P., Hosoo,Y., Goshima,S., Horie,T., Eckelman B., Yamada,K., Yoshida,K., Bakker,EP., Shinmyo,A., Oiki,S., Schroeder,J.I., and Uozumi,N.: "Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants."Proc.Natl.Acad.Sci.USA.. 99. 6428-6433 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Maser,P., Eckelman,B., Vaidyanathan,R., Fairbain,D.J., Kubo,M., Yamagami,M., Yamaguchi,K., Nishimura,M., Uozumi,N., Robertsin,W., Sussman,M.R., and Schroeder,J.I.: "Altered shoot/root Na^+ distribution and bifurcating salt sensitivity in Arabidopsis by genetic disruption of the Na^+ transporter AtHKTl"FEBS Letters. 531. 157-161 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Sato,Y., Hosoo,Y., Sakaguchi,M., and Uozumi,N.: "(2003) Requirement of negative residues, Asp 95 and Asp 105, in S2 on channel function and membrane integration of voltage-dependent K^+ channel, KATl"Biosci.Biotech.Biochem.. 67. 923-926 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sato,Y., Sakaguchi,M., Goshima,S., Nakamura,T., and Uozumi,N.: "Molecular dissection of the contribution of negatively and positively charged residues in S2, S3, and S4 to the final membrane topology of the voltage sensor in the K^+ channel, KATl"J.Biol.Chem.. 278. 13227-13234 (2003)
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「研究成果報告書概要(欧文)」より
