2003 Fiscal Year Final Research Report Summary
Analyses of growth performance by fluorescent substances and trace elements in mollusks
| Project/Area Number |
13660189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Ishinomaki Senshu University |
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Principal Investigator |
OKOSHI Kenji Ishinomaki Senshu University, Department of Biotechnology, Associate Professor, 理工学部, 助教授 (60201969)
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| Project Period (FY) |
2001 – 2003
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| Keywords | shell / age determination / growth analysis / trace element / fluorescent substance / strontium chloride / tetracycline / bivalve |
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| Research Abstract |
Biomineralization research is one of the more important research subjects from both a fundamental and applied point of view. The molluscan shells contain considerable information about their own growth history including the continuous condition of mineralization and stress or disease. Analysis of this information is a factor of great importance both in marine biology and in aquaculture. The application of fluorescent substances to the analysis of growth performance and hard tissue formation is popular in medical and dental sciences. The fish otoliths also can be marked by immersion in some fluorescent substances. The object of this study is to develop a method of marking the hard tissues of mollusks such as shell and pearl of bivalves with fluorescent substances in order to obtain effective information on hard tissue formation. To determine the optimum tetracycline (TC) and alizarine complexone (ALC) concentration and treatment time for marking the shells of oyster Crassostrea gigas various combinations of concentration and treatment time were tested. TC presented a clear yellow fluorescent line in the dorso-ventral section of oyster shells under UV light. The optimum condition for treatment was 200mg/l for 12-24 h. Orange mark was observed by ALC treatment at 150-300mg/l for 12-24 h. Double treatments by TC and by ALC were successful. Similarity and difference of growth performance between the shell and the pearl can be observed in the pearl oyster Pinctada fucata. Because the treatment had no detectable deleterious effects on the growth and survival of the animals studied, the application of the method to the marine mineralized samples such as coccolithophores, reef-building corals and mollusks should promise the development not only in marine biology but also in aquaculture.
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