2002 Fiscal Year Final Research Report Summary
Differentiation of immature enterocytes into enteroendocrine cells by Pdx-1 overexpression.
| Project/Area Number |
13670010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Shiga University of Medical Sgience |
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Principal Investigator |
FUJIMIYA Mineko Dept.Anatomy, Shiga Univ.Med.Sci., 医学部, 助教授 (10199359)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Takaaki Dept.Anatomy Shiga Univ.Med. Sci., 医学部, 助手 (30314157)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Intestinal Stem cell / IEC6 cell / Pdx-1 / transcriptinal factor / enteroendocrine cells / Pancreatic B cells |
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| Research Abstract |
The development of a variety of enteroendocrine cells of the gut is poorly understood. We tested whether immature intestinal stem cells were switched to multiple enteroendocrine hormone-producing cells by in vitro transfer of a homeobox gene. We transfected the pancreatic-duodenal homeobox 1 gene (Pdx1) into IEC-6 cells, an embryonic intestinal epithelial cell line derived from a normal rat, and selected the cells that overexpressed Pdx1 by 150-fold compared with control. The cells were examined for differentiation into enteroendocrine cells by immunocytochemical and electron microscopic analyses. Transfected cells cultured on micropore filters formed a trabecular network piled up on monolayer cells. These trabecular cells showed nuclear localization of Pdx1 protein and contained well-developed rough endoplasmic reticulum as well as many secretory granules of pleomorphic shape in the cytoplasm. Antibodies against chromogranin A, serotonin, cholecystokinin, gastrin, and somatostatin stained these secretory granules in the cytoplasm. Furthermore, immunofluorescence double staining analysis showed that different hormones were produced within a cell. These results provide the evidence that immature intestinal epithelial cells can differentiate into multiple hormone-producing enteroendocrine cells in response to overexpression of Pdx1.
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Research Products
(27 results)
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[Publications] A.Asakawa, A.Inui, T.Kaga, H.Yuzuriha, T.Nagata, N.Ueno, S.Makino, M.Fujimiya, A.Niijima, M.Fujino, M.Kasuga: "Ghrelin is an appetite-stimulatory signal from stomach with structural resemblance to motilin"Gastroenterology. 120. 337-345 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] T.Nakamura, A.Kishi, Y.Nishio, H.Maegawa, K.Egawa, N.C.W.Wong, H.Kojima, M.Fujimiya, R.Arai, A.Kashiwagi, R.Kikkawa: "Insulin production in a neuroectodermal tumor that expresses ISL-1 but not Pdx1"J.Clin.Endocr.Metab.. 86. 1795-1800 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] T.Kaga, A.lnui, M.Okita, A.Asakawa, N.Ueno, M.Kasuga, M.Fujimiya, N.Nishimura, R.Dobashi, Y.Morimoto, I-M.Liu, J-T.Cheng: "Modest overexpression of neuropeptide Y in the brain leads to obesity after high sucrose feeding"Diabetes. 50. 1206-1210 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] H.Kojima, T.Nakamura, Y.Fujita, A.Kishi, M.Fujimiya, S.Yamada, M.Kudo, Y.Nishio, H.Maegawa, M.Haneda, H.Yasuda, I.Kojima, M.Seno, NC Wong, R.Kikkawa, A.Kashiwagi: "Combined expression of pancreatic duodenal homeobox 1 and islet factor 1 induces immature enterocytes to produce insulin"Diabetes. 51. 1398-1408 (2002)
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「研究成果報告書概要(欧文)」より
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