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2002 Fiscal Year Final Research Report Summary

Histochemical and functional aspects of glycolipids

Research Project

Project/Area Number 13670024
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyorin University

Principal Investigator

KAWAKAMI Hayato  Department of Anatomy, Kyorin University School of Medicine, Professor, 医学部, 教授 (30146542)

Project Period (FY) 2001 – 2002
Keywordsglycolipid / ganglioside / globoside / monoclonal antibodies / histochemistry / Purkinje cell / neurotransmitter / intestine
Research Abstract

Histochemical localization of various kinds of glycolipids was investigated with the use of confocal laser scanning microscopy and transmission electron microscopy. Rat various organs prefixed with 4% formaldehyde and 1% glutaraldehyde were cut into nonfrozen slices (40μm thick) and treated with monoclonal antibodies against glycolipids (anti-GM1, GD2, GD3, GT1b, GQ1b, Gb3 etc.) followed by treatment with Cy3-labeled socondary antibody for light microscopy or peroxidase-labeled socondary antibody for electron microscopy. Some of the specimens from neuronal organs were also stained with specific antibodies against neurotransmitters (GABA, glutamate etc.) by Cy5 labeling method. In cerebellar cortex, each ganglioside revealed specific distribution pattern, e.g. anti-GQ1b antibody was positive for the plasma membrane of Purkinje cell body and its dendrites, Golgi cisternae, part of the nerve endings around the Purkinje cell body. These areas especially the nerve endings around the Purkinje cell body were also positive for anti-GABA antibody. In small intestine, Auerbach's plexus and outer muscular layer were specifically stained for some ganglioside antibodies e.g. GM1, GD2. Basolateral plasma membrane of intestinal gland epithelial cells were specifically stained for anti-Gb3. Specific distribution of glycolipids in various organs seem to have some specific relationships with neuronal functions and other cellular functions.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 川上 速人: "糖脂質の電顕組織化学"電子顕微鏡. 37. 215-217 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka, H.: "Importance of N-linked sugar residues in the developement of Auerbach's plexus in the rat colon : a lectin histochemical study"Histochem. J.. 37. 215-217 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akimoto, Y.: "Changes in Distribution of the Long Form of Type XII Collagen during Chicken Corneal Development"J. Histochem. Cytochem.. 50. 851-862 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasaki, T.: "Distribution of the Sialoglycoconjugates in the Rat Cerebellum and Its Change after Aging"J. Histochem. Cytochem.. 50. 1179-1186 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawakami, H.: "Myosin light chain phosphorylation is correlated with cold-induced changes in platelet shape"J. Smooth Muscle Res.. 37. 113-122 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, Y.: "Location of sialoglycoconjugates containing the Siaα2-3 Gal and Siaα2-6 Gal groups in the rat hippocampus and the effect of aging on their expression"J. Histochem. Cytochem.. 49. 1311-1319 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 牛木 辰男: "走査電顕アトラス マウスの発生"岩波書店. 298 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato Y., Akimoto Y., Kawakami H., Hirano H., Endo T.: "Location of sialoglycoconjugates containing the Sia α2- 3Gal and Sia α2-6Gal groups in the rat hippocampus and the effect of aging on their expression"J Histochem Cytochem. 49. 1311-1319 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyata H., Kawakami H., Hori T., Hirano H.: "N-linked oligosaccharides predominantly distribute in rat cerebellar cortex"Okajimas Folia Anat Jpn. 78. 129-140 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kawakami H., Higashihara M., Ohsaka M., Miyazaki K., Ikebe M., Hirano.: "Myosin light chain phosphorylation is correlated with cold-induced changes in platelet shape"J Smooth Muscle Res. 37. 113-122 (2001)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Shindo T., Kurihara Y., Nishimatsu H., Moriyama N., Kakoki M., Wang Y., Imai Y., Ebihara A., Kuwaki T., Ju KH., Minamino N., Kangawa K., Ishikawa T., Fukuda M., Akimoto Y., Kawakami H., et al.: "Vascular abnormalities and elevated blood pressure in mice lacking adrenomedullin gene"Circulation. 104. 1964-1971 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Usui T., Hara M., Satoh H., Moriyama N., Kagawa H., Amano S., Ohika T., Ishii Y., Ibaraki N., Hara C., Kunimi M., Noiri E., Tsukamoto K., Inatomi J., Kawakami H., et al.: "Molecular basis of oular abnormalities associated with proximal renal tubular acidosis"J Clin Invest. 108. 107-115 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka S., Kudo A., Hirano H., Kawakami H., Kuwano T., Higashihara E., Tanaka H., Delarue F., Sraer J-D., Mune T., Krozowski ZS., Yan K.: "11b-hydroxysteroid dehydrogenase type 2 is expressed in the human kidney glomerulus"J Clin Endocrinol Metab. 87. 877-882 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Noma T., Kanai Y., Kanai-Azuma M., Ishii M., Fujisawa M., Kurohmaru M., Kawakami H., Wood SA., Hayashi Y.: "Stage- and sex-dependent expressions of Usp9x, and X-linked mouse orthologue of Drosophila Fat facets, during gonadal development and oogenesis in mice"Gene Exp Patterns. 2. 87-91 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Shindo T., Manabe I., Fukushima Y., Tobe K., Aizawa K., Miyamoto S., Kawai-Kowase K., Moriyama N., Imai Y., Kawakami H., et al.: "Krupel-like zinc-finger transcription factor KLF5/BTEB2 is a target for angiotensin II signaling and an essential regulator of cardiovascular remodeling"Nature Med. 8. 856-863 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aizaki H., Harada T., Otsuka M., Seki N., Matsuda M., Li YW., Kawakami H., Matsuura Y., Miyamura T., Suzuki T.: "Expression profiling of liver cell lines expressing entire- or parts of hepatitis C virus open reading frame"Hapatology. 36. 1431-1438 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki T., Akimoto Y., Sato Y., Kawakami H., Hirano., Endo T.: "Distribution of the sialogycoconjugates in the rat cerebellum and its change after aging"J Histochem Cytochem. 50. 1179-1186 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akimoto Y., Yamakawa N., Furukawa K., Kimata K., Kawakami H., Hirano H.: "Changes in distribution of the long form of Type XII collagen during chicken corneal development"J Histochem Cytochem. 50. 851-862 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka H., Ito Y., Nirasawa Y., Kawakami H., Hirano H.: "Importance of N-linked sugar residues in the development of Auerbach's plexus in the rat colon: a lectin histochemical study"Histochem J. 34. 11-116 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawakami H.: "Immuno-electron microscopy of glycolipids, (in Japanese)"Electron Microscopy. 37. 215-217 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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