2002 Fiscal Year Final Research Report Summary
Elucidation of the molecular functions of paxillin in cell cycles
| Project/Area Number |
13670025
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Keio University |
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Principal Investigator |
AISO Sadakazu Keio University, School of Medicine, Department of Anatomy, Professsor, 医学部, 教授 (60138013)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Motoyuki Keio University, School of Medicine, Department of Anatomy, Fellow, 医学部, 助手 (90255422)
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| Project Period (FY) |
2001 – 2002
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| Keywords | paxillin / xenopus / cell cycle |
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| Research Abstract |
Paxillin has been recognized as a focal adhesion adapter protein that participates in the integrin-mediated signaling. An earlier study [Ogawa et al.Biochim.Biophys.Acta 1519 (2001) 235] found that frog paxillin was expressed in the kidney epithelial cell line A6 and localized in the nucleus. Here, in this study, we have found that the expression of frog paxillin is up-regulated in the S phase of cell cycle. The protein became phosphorylated on tyrosine when the cells were grown on vitronectin ; the tyrosine phosphorylation was not detectable when the cells were cultured on fibronectin, laminin or poly-D-lysine. On the other hand, MAPkinase was revealed to phosphorylate frog paxillin on serine. Both phosphorylation events, namely on tyrosine and serine, were essential for the nuclear translocation of this protein.
Our results suggest that the integrin-mediated signaling pathway and the MAP kinase pathway meet at paxillin.
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[Publications] Nogami H, Hiraoka Y, Matsubara M, Nonobe E, Harigaya T, Katayama M, Hemmi N, Kobayashi S, Mogi K, Also S, Kawamura K, Hisano S.: "A composite hormone response element regulates transcription of the rat GHRH receptor gene"Endocrinology. l43(4). 1318-1326 (2002)
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[Publications] Tajima H, Niikura T, Hashimoto Y, Ito Y, Kita Y, Terashita K, Yamazaki K, Koto A, Aiso S, Nishimoto I.: "Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults"Neurosci Lett.. 324(3). 227-231 (2002)
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[Publications] Hashimoto Y, Tsuji O, Niikura T, Yamagishi Y, Ishizaka M, Kawasumi M, Chiba T, Kanekura K, Yamada M, Tsukamoto E, Kouyama K, Terashita K, Aiso S, Lin A, Nishimoto I.: "Involvement of c-Jun N-terminal kinase in amyloid precursor protein-mediated neuronal cell death"J Neurochem.. 84(4). 864-877 (2003)
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[Publications] Niikura T, Hashimoto Y, Tajima H, Ishizaka M, Yamagishi Y, Kawasumi M, Nawa M, Terashita K, Aiso S, Nishimoto I.: "A tripartite motif protein TRIM 11 binds and destabilizes Humanin, a neuroprotective peptide against Alzheimer's disease-relevant insults"Eur J Neurosci.. l7(6). 1150-1158 (2003)
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