2001 Fiscal Year Annual Research Report

線溶系因子による細胞運動調節機構の細胞表面分子間反応の可視化による解析

Research Project

Project/Area Number	13670040
Research Institution	Hamamatsu University School of Medicine
Principal Investigator	浦野哲盟浜松医科大学, 医学部, 教授 (50193967)
Co-Investigator(Kenkyū-buntansha)	山本清二浜松医科大学, 光量子医学研究センター, 助教授 (60144094) 井原勇人浜松医科大学, 医学部, 助手 (00223298)
Keywords	細胞接着 / 細胞移動 / ウロキナーゼ受容体 / ウロキナーゼ / PAI-1 / PAI-2
Research Abstract	urokinase type plasminogen activator(uPA)は癌細胞表面や新生血管内皮細胞上の特異受容体(uPAR)に結合しており、そのインヒビターであるPA inhibitor 1(PAI-1)も腫瘍組織に多く存在する。本研究はこれらの機能蛋白の分子間相互反応を可視化し、腫瘍細胞の移動や血管新生時の細胞運動における役割を明らかにすることを目的とする。本年度の研究において以下の結果が得られた。 uPAR及びuPAを発現しているhuman sarcoma cell lineであるHT1080を実験に用いた。HT1080はfibronectin(FN)に比較して、Vitronectin(Vn)-coatのdishに良く接着した。Vn-coat dishとの接着はactive recombinant human PAI-1により濃度依存性に阻害された。しかしFNあるいはcollagen-coatのdishへの接着は阻害されなかった。また活性潜在型(latent)PAI-1及びPAI-2はいずれも阻害しなかった。Vnからcollagenへの細胞移動はactive PAI-1により濃度依存性に促進されたが、PAI-2は影響しなかった。現在Grenn Fluorescent Protein(GFP)標識uPAR及びFITC標識uPAを用いてエヴァネッセント蛍光顕微鏡で細胞移動時のこれらの標識蛋白の局在の変化を解析中である。

Research Products
(12 results)

All Other

All Publications (12 results)

[Publications] Ihara, H. et al.: "Induction of plasminogen activator inhibitor-1 (PAI-1) gene expression in adipocytes by thiazolidinediones"FASEB J.. 15・7. 1233-1235 (2001)
[Publications] Urano T. et al.: "The expression of exercise-induced tPA activity in blood is regulated by the basal level of PAI-1"Thromb. Haemost.. 85. 751-752 (2001)
[Publications] Nagai N. et al.: "Tissue-type plasminogen activator enhances neuronal death induced by oxygen-glucose-deprivation in culture"J Cereb Blood Flow Metab. 21・6. 631-634 (2001)
[Publications] Urano T. et al.: "The profibrinolytic enzyme subtilisin NAT purified from Bacillus subtilis cleaves and inactivates plasminogen activator inhibitor type 1"J. Biol. Chem.. 276・27. 24690-24696 (2001)
[Publications] Takahashi H. et al.: "Dissociation of systemic and hippocampal modulation of rat locomotor activity by 5-HT(2C) receptors"Neurosci Res.. 40・1. 97-103 (2001)
[Publications] Konno H. et al.: "Urokinase receptor and vascular endothelial growth factor are synergistically associated with the liver metastasis of colorectal cancer"Jpn J Cancer Res.. 92・5. 516-523 (2001)
[Publications] Pawlak R. et al.: "The differential effect of angiotensin II and angiotensin 1-7 on norepinephrine, epinephrine, and dopamine concentrations in rat hypothalamus : the involvement of angiotensin receptors"Brain Res Bull.. 54・6. 689-694 (2001)
[Publications] Yoshino A. et al.: "Enhanced secretion of tissue plasminogen activator by simultaneous use of retinoic acid and ascorbic acid from tissue cultured gastroepiploic artery"Life Sciences. 70. 1461-1470 (2002)
[Publications] Aoki K et al.: "Elevation of plasma free PAI-1 levels as an integrated endothelial response to sever burs"Burns. 27. 569-575 (2001)
[Publications] Pawlak R. et al.: "Rapid, specific and active site-catalyzed effect of tissue-plasminogen activator on hippocampus-dependent learning in mice"Neuroscience. (in press).
[Publications] Yoshida M. et al.: "L-158,809 and (D-Ala^7)-angiotensin I/II (1-7) decrease PAI-1 release from human umbilical vein endothelial cells"Thromb. Res.. (in press).
[Publications] Nagai N. et al.: "Tissue-type plasminogen activator has paradoxical roles in focal cerebral ischemic injury by thrombotic middle cerebral artery occlusion with mild or severe photochemical damage in mice"J Cereb Blood Flow Metab.. (in press).

2001 Fiscal Year Annual Research Report

線溶系因子による細胞運動調節機構の細胞表面分子間反応の可視化による解析

Principal Investigator

浦野 哲盟 浜松医科大学, 医学部, 教授 (50193967)

Research Products

[Publications] Ihara, H. et al.: "Induction of plasminogen activator inhibitor-1 (PAI-1) gene expression in adipocytes by thiazolidinediones"FASEB J.. 15・7. 1233-1235 (2001)

[Publications] Urano T. et al.: "The expression of exercise-induced tPA activity in blood is regulated by the basal level of PAI-1"Thromb. Haemost.. 85. 751-752 (2001)

[Publications] Nagai N. et al.: "Tissue-type plasminogen activator enhances neuronal death induced by oxygen-glucose-deprivation in culture"J Cereb Blood Flow Metab. 21・6. 631-634 (2001)

[Publications] Urano T. et al.: "The profibrinolytic enzyme subtilisin NAT purified from Bacillus subtilis cleaves and inactivates plasminogen activator inhibitor type 1"J. Biol. Chem.. 276・27. 24690-24696 (2001)

[Publications] Takahashi H. et al.: "Dissociation of systemic and hippocampal modulation of rat locomotor activity by 5-HT(2C) receptors"Neurosci Res.. 40・1. 97-103 (2001)

[Publications] Konno H. et al.: "Urokinase receptor and vascular endothelial growth factor are synergistically associated with the liver metastasis of colorectal cancer"Jpn J Cancer Res.. 92・5. 516-523 (2001)

[Publications] Pawlak R. et al.: "The differential effect of angiotensin II and angiotensin 1-7 on norepinephrine, epinephrine, and dopamine concentrations in rat hypothalamus : the involvement of angiotensin receptors"Brain Res Bull.. 54・6. 689-694 (2001)

[Publications] Yoshino A. et al.: "Enhanced secretion of tissue plasminogen activator by simultaneous use of retinoic acid and ascorbic acid from tissue cultured gastroepiploic artery"Life Sciences. 70. 1461-1470 (2002)

[Publications] Aoki K et al.: "Elevation of plasma free PAI-1 levels as an integrated endothelial response to sever burs"Burns. 27. 569-575 (2001)

[Publications] Pawlak R. et al.: "Rapid, specific and active site-catalyzed effect of tissue-plasminogen activator on hippocampus-dependent learning in mice"Neuroscience. (in press).

[Publications] Yoshida M. et al.: "L-158,809 and (D-Ala^7)-angiotensin I/II (1-7) decrease PAI-1 release from human umbilical vein endothelial cells"Thromb. Res.. (in press).

[Publications] Nagai N. et al.: "Tissue-type plasminogen activator has paradoxical roles in focal cerebral ischemic injury by thrombotic middle cerebral artery occlusion with mild or severe photochemical damage in mice"J Cereb Blood Flow Metab.. (in press).

浦野哲盟浜松医科大学, 医学部, 教授 (50193967)