2002 Fiscal Year Final Research Report Summary
生体機能調節におけるコレシストキニン(CCK)-A,-B受容体の機能分担と遺伝子の発現 副題:自然発症CCK-A受容体欠損ラット、CCK-A,-B受容体遺伝子ターゲティングマウスによる検討
| Project/Area Number |
13670077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
KANAI Setuko Tokyo Metro. Inst. Gerontol. Head of Dept. Clin. Physiol., ASSISTANTS RESEARCHER, 東京都老人総合研究所・生体機能調節と加齢グループ, 研究助手 (90100122)
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| Co-Investigator(Kenkyū-buntansha) |
MIYASAKA Kyouko Tokyo Metro. Inst. Gerontol. Head of Dept. Clin. Physiol., HEAD, 福祉振興財団東京都老人総合研究所・生体機能調節と加齢グループ, グループリーダー (90166140)
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| Project Period (FY) |
2001 – 2002
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| Keywords | CCK receptor / CCK-B receptor / CCK-A receptor / pancreas / gene / gene expression / gallstone |
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| Research Abstract |
Two types of CCK receptors (type A and type B) have been identified. We raised CCK-A,-B and AB receptor knockout mice, the role of these receptors in gastric functions and bile-pancreatic secretions was determined.
1. Basal gastric acid secretion was decreased in mice lacking CCK-BR. Administration of gastrin and histamine significantly increased acid secretion in all genotypes.
2. Lack of CCK-BR enhanced gastric emptying of a liquid load. The gastric emptying was inhibited by administration of CCK in mice with CCK-AR, but not in mice without CCK-AR. Atropine inhibited gastric emptying in all genotypes, however, larger doses were required to reveal a similar effect in mice with CCK-BR to in mice without CCK-BR. Therefore, autonomic nerve functions might be altered by the lack of CCK-BR
3. We confirmed that CCK did not induce gallbladder contraction nor pancreatic amylase secretion in CCK-AR(-/-) mice. Other stimulants such as acethocholine and neuromedin C could stimulated amylase secretion in CCK-AR(-/-) mice but could not produce gallbladder contraction. Secretin failed to increase bicarbonate secretion in mice, and CCK increased bicarbonate secretion via CCK-A receptor in mice. Glucose tolerance was not altered in CCK-AR(-/-) mice.
4. Age associated gallstone formation was induced by administration of a higher protein and fat diet in CCK-AR(-/-) mice, but not in wild type mice.
5. Anxiety related behaviors in the plus maze were increased in mice without CCK-BR.
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Research Products
(23 results)
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[Publications] Miyasaka K, Kobayashi S, Ohta M, Kanai S, Yoshida Y, Nagata A, Matsui T, Noda T, Takiguchi S, Takata Y, Kawanami T, Funakoshi A: "Anxiety-related behaviors in cholecystokinin-A,B, and AB receptor gene knockout mice in the plus-maze"Neurosci Lett. 335. 115-118 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Takiguchi S, Suzuki S, Sato Y, Kanai S, Miyasaka K, Jimi A, Shinozaki H, Takata Y, Funakoshi A, Kono A, Minowa O, Kobayashi T, Noda T: "Role of CCK-A receptor for pancreatic function in mice : A study in CCK-A receptor knockout mice"Pancreas. 24. 276-283 (2002)
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[Publications] Miyasaka K, Ichikawa M, Ohta M, Kanai S, Yoshida Y, Masuda M, Nagata A, Matsui T, Noda T, Takiguchi S, Takata Y, Kawanami T, Funakoshi A: "Increased energy metabolism and energy turnover in mice lacking cholecystokinin-B receptor"J Nutr. 132. 739-741 (2002)
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「研究成果報告書概要(欧文)」より
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