2003 Fiscal Year Final Research Report Summary
Function of tyrosine phosphorylation of BIT in photic entrainment of the circadian clock in rats
| Project/Area Number |
13670122
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
OKUMURA Nobuaki Osaka Univ., Institute for Protein Research, Associate Prof., 蛋白質研究所, 助教授 (20224173)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Circadian rhythm / BIT / SHIPS-1 / Hypothalamus / Tyrosine phosphorylation |
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| Research Abstract |
Circadian rhythms of mammals are generated by a circadian oscillation of master pacemaker genes in the suprachiasmatic nucleus of the hypothalamus (SCN), and entrained by environmental factors such as 24-h light-dark cycles. We have previously shown that light exposure during the dark period enhanced tyrosine phosphorylation of brain immunoglobulin-like molecule with tyrosine-based activation motifs (BIT) in the rat SCN. Our major findings from last year were as follows, (1) BIT was tyrosine phosphorylated in response to light in the retina, (2) BIT was tyrosine phosphorylated on cold exposure in various nuclei of the hypothalamus, (3) BIT was associated with pl37GPI-anchored protein and clathrin heavy chain, (4) BIT was internalized by endocytosis and this process might be regulated by its tyrosine phosphorylation.
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