2002 Fiscal Year Final Research Report Summary
Expression of galectin-3 and its clinical application to preoperative cytological diagnosis in thyroid neoplasms
| Project/Area Number |
13670180
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
NAKANO Shizuo Kagoshima University, University Hospital, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (00295255)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YONEZAWA Suguru Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (10175002)
YOSHINAKA Heiji Kagoshima University, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (80191625)
AIKOU Takashi Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (60117471)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Keywords | Galectin-3 / Thyroid neoplasm / Preoperative diagnosis |
|---|
| Research Abstract |
Galectin-3 is a member of the β-galactoside-binding protein family which plays an important role in cell-cell adhesion and in cell-matrix interaction and is expressed in many neoplastic lesion. It is reported that Galectin-3 influences to the progression and metastasis. We examined expression of galectin-3 in various thyroid neoplasms. Expression of galectin-3 was evaluated in immunohistochemically stained specimen using anti-galectin-3 antibody. A galectin-3 staining score was calculated by using stained area and concentration. Mean staining score was respectively 0.061, 0.427, and 1.514 in 69 lesions of follicular adenomas, 62 of follicular carcinomas, and 115 of papillary carcinomas. Staining score was significantly higher in the follicular carcinomas than in the follicular adenomas and was significantly higher in the papillary carcinomas than in the follicular adenomas or carcinomas.
Furthermore, primary lesion of papillary carcinomas with lymph node metastases expressed higher concentration of galectin-3 than that without metastases. Expression of galectin-3 was rarely found in normal thyroid tissues. Galectin-3 expression was significantly different in 3 histological types. From these results, we assumed that galectin-3 works in different ways at different stages of thyroid neoplasm proliferation.
Moreover, we examined expression of galectin-3 in a stump cytology specimen of thyroid tumor. Galectin-3 was also well expressed in cytological specimens as same as paraffin-bedded specimens. Therefore, there is a possibility that expression of galectin-3 is applicable to use for preoperative cytological diagnosis of thyroid gland tumor.
|
