2002 Fiscal Year Annual Research Report

腺管分離法を用いた大腸腫瘍のDNA multiploidの遺伝子解析

Research Project

Project/Area Number	13670187
Research Institution	Iwate Medical University
Principal Investigator	菅井有岩手医科大学, 医学部, 助教授 (20187628)
Keywords	大腸癌 / 大腸腺腫 / APC / DNA ploidy / Ki-ras / LOH / MSI / p53
Research Abstract	我々は腺管分離法を用いて進行大腸癌のDNA ploidyと遺伝子異常との関係を検討してきた。それらの結果からDNA diploid(D)、aneuploid(A)、multiploid(M, D+A)は互いに異なるgenetic plofileを有する独立したDNA typeであることを明らかにした(Sugai T, et al. Hum Pathol, 2000;Sugai T, et al. Int J Cancer, 2000;Sugai T, et al. Oncogene, 2001;Sugai T, et al. Cytometry 2001;46:345-350.)。上記のDNA typeはDNA index(DI)からも異なっており、Aは1.3未満のlow DIで構成されているのに対して、Mは1.3以上のhigh DIで構成されていた(Sugai T, et al. Cytometry 2001;46:345-350.)。一方大腸腺腫のaneuploid stateのDIは1.3未満のlow DI腺腫と1.5以上のhigh DI腺腫に2大別されるが(Sugai T et al. Pathol Int 1999;49:617-625.)、早期癌の場合、腺腫内癌(group A cancer)と腺腫成分を含まないかもしくは極めて少量含む癌(group B cancer)に大別してDNA ploidy stateを検討した場合、前者の癌はlow DIで癌成分が構成されているのに対して、後者はlow及びhigh DI癌細胞で構成されていることが明らかになった(Sugai T et al. Pathol Int 2003;53:154-162)。これらの検討から大腸腫瘍の新しい腫瘍発生を提案した。

Research Products

(6 results)

All Other

All Publications (6 results)

[Publications] Suto T, Sugai T, et al.: "Allelotype analysis of the PTEN, Smad 4 and DCC genes in biliary tract cancer"Anticancer Res. 22. 1529-1536 (2002)
[Publications] Sugai T, et al.: "Molecular validation of the modified Vienna classification of colorectal tumors"J Mol Diagn. 4. 191-200 (2002)
[Publications] Takahashi H, Sugai T, et al.: "Application of the crypt isolation technique to the assessment of genetic alterations of colorectal carcinomas"Pathol Int. 52. 628-635 (2002)
[Publications] Sugai T, et al.: "Analysis of genetic alterations, classified according to their DNA ploidy pattern, in the progression of colorectal adenomas and early colorectal carcinomas"J Pathol. (in press).
[Publications] Sugai T, et al.: "Evolution of DNA ploidy state and DNA index in colorectal adenomas and carcinomas using the crypt isolation technique -a new hypothesis in colorectal tumorigenesis"Pathol Int. 53. 154-162 (2003)
[Publications] Habano W, Sugai T, et al.: "Reduced expression and loss of lieterozygisity of the SDHD gene in colorectal and gastric cancer"Oncol Rep. (in press).

2002 Fiscal Year Annual Research Report

腺管分離法を用いた大腸腫瘍のDNA multiploidの遺伝子解析

Principal Investigator

菅井 有 岩手医科大学, 医学部, 助教授 (20187628)

Research Products

[Publications] Suto T, Sugai T, et al.: "Allelotype analysis of the PTEN, Smad 4 and DCC genes in biliary tract cancer"Anticancer Res. 22. 1529-1536 (2002)

[Publications] Sugai T, et al.: "Molecular validation of the modified Vienna classification of colorectal tumors"J Mol Diagn. 4. 191-200 (2002)

[Publications] Takahashi H, Sugai T, et al.: "Application of the crypt isolation technique to the assessment of genetic alterations of colorectal carcinomas"Pathol Int. 52. 628-635 (2002)

[Publications] Sugai T, et al.: "Analysis of genetic alterations, classified according to their DNA ploidy pattern, in the progression of colorectal adenomas and early colorectal carcinomas"J Pathol. (in press).

[Publications] Sugai T, et al.: "Evolution of DNA ploidy state and DNA index in colorectal adenomas and carcinomas using the crypt isolation technique -a new hypothesis in colorectal tumorigenesis"Pathol Int. 53. 154-162 (2003)

[Publications] Habano W, Sugai T, et al.: "Reduced expression and loss of lieterozygisity of the SDHD gene in colorectal and gastric cancer"Oncol Rep. (in press).

菅井有岩手医科大学, 医学部, 助教授 (20187628)