2002 Fiscal Year Final Research Report Summary
Molecular analysis of chromosome breakpoints in human chromosome 1 specific for malignant lymphoma
| Project/Area Number |
13670206
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATOH Hitoshi The Institute of Medical Science, Research Associate, 医科学研究所, 助手 (70183829)
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| Project Period (FY) |
2001 – 2002
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| Keywords | 1p36 translocation / malignant lymphoma / FISH / gene amplification |
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| Research Abstract |
We have previously arrayed 21 cosmid and five P1 phage clones along with the short arm of chromosome 1 from the telomere to the centromere direction by pair-wise comparison experiment using multi-color FISH technique. In reference to this physical map covering 1p36 region, we assessed the breakpoints in three of in vitro established cell lines and two of in vivo cell lines maintained in SCID mice. As a result, all of the five cell lines had different breakpoints. The breakpoint of HMS, an in vivo cell line maintained in SCID mice, was mapped between markers SHGC-110595 and D1S1615 spanning about 260-kb long within the 1p36 region. Narrowing the possible breakpoint is still on going.
On the other hand, we have established two independent cell lines from patients of primary effusion lymphomas(PELs) both of which contained marker chromosomes consisted with an insertional chromosome fragment derived from chromosome 1. Comparative genomic hybridization(CGH) analysis revealed that the chromosomal region of 1q25 is amplified commonly. Moreover, the pattern of this amplification unit in PSu cell line was identified as a B-A-C-A-B type by FISH using cosmid probes. The size of amplification unit is not yet known, and what kind of genes are located at the border of this unit and associated with this effusion type of B-cell lymphoma is remained to study.
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[Publications] Fukami, T., Satoh. H., Fujita, E., Maruyama, T., Fukuhara, H., Kuramochi, M., Takamoto, S., Momoi, T. and Murakami, Y.: "Identification of the Tslc 1 gene, a mouse orthologue of the human tumor suppressor TSLC1 gene"Gene. 295. 7-12 (2002)
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[Publications] Endo, H., Takenaga, K., Kanno, T., Satoh, H., and Mori, S.: "Methionine aminopeptidase 2 is a new target for the metastasis-associated protein, S100A4*"J. Biol. Chem.. 277. 26396-26402 (2002)
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[Publications] Kakiuchi, C., Ishida, T., Sato, H., Katano, H., Ishiko, T., Mukai, H., Kogi, M., Kasuga, N., Takeuchi, K., Yamane, K., Fukayama, M. and Mori, S.: "Secretion of interleukin-6 and vascular endothelial growth factor by spindle cell sarcoma complicating Castleman's disease (so-called 'Vascular neoplasia')"J. Pathol.. 197. 264-271 (2002)
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[Publications] Maekawa, I., Satoh, H., Aoki, N., Morishita, Y., Tsukamoto, N., Karasawa, M., Nonaka, Y., Shiota, M., Nojima, Y. and Mori, S.: "Maintenance and characterization of an Epstein Barr virus-infected CD56-negative T cell lymphoma"Jpn. J. Cancer Res.. 93. 61-69 (2002)
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[Publications] Fujino, Y., Mizuno, T., Masuda, K., Ohno, K., Satoh, H. and Tsujimoto, H.: "Assignment of the feline Fas (TNFRSF6) gene to chromosome D2p13-p12.2 by fluorescence in situ hybridization"Cytogenet. Cell Genet.. 95. 122-124 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Wang, P., Abe, K., Ojima, T., Ohyashiki, JH., Satoh, H., Maruyama, T., Nagata, H., Tanaka, H. and Yamamoto, K.: "3'-fluorine-substitution in 3'-fluorocarbocyclic oxetanocin a augments the drug's inhibition of HHV-6B propagation in chronically infected cultures"Microbiol. Immunol.. 45. 457-466 (2001)
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[Publications] Fujino, Y., Mizuno, T., Masuda, K., Ohno, K., Satoh, H. and Tsujimoto, H.: "Assignment of the feline Fas ligand gene (TNFSF6) to chromosome F1q12-q13 by fluorescence in situ hybridization"Cytogenet. Cell Genet.. 94. 92-93 (2001)
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[Publications] Moriyama, M., Tsukamoto, Y., Fujiwara, M., Kondo, G., Nakada, C., Baba, T., Ishiguro, N., Miyazaki, A., Nakamura, K., Hori, N., Sato, K., Shomori, K., Takeuchi, K., Satoh, H., Mori, S. and Ito, H.: "Identification of a novel human ankyrin-repeated protein homologous to CARP"Biochem. Biophys. Res. Comm.. 285. 715-723 (2001)
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