2003 Fiscal Year Final Research Report Summary
Molecular pathological study on tumorigenesis of beta-catenin gene alterated foci in rat colon mucosa
| Project/Area Number |
13670208
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
YOSHIMI Naoki YOSHIMI,Naoki, 医学部, 教授 (30166996)
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| Co-Investigator(Kenkyū-buntansha) |
MORIOKA Takamitsu University of the Ryukyus, Faculty of Medicine, Associate, 医学部, 助手 (70253961)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Rat / Colon Carcinogenesis / beta-catenin / Chemoprevention / Preneoplastic lesions |
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| Research Abstract |
Although beta-catenin accumulated crypts(BCAC)is an useful biomarker in the retrieval of the chemopreventive agents on rat colon carcinogenesis, the detection of BCAC is complicated, since the preparation manufacture is needed.In this study, it was examined the mucin or mucoprotein in neoplastic lesions using the High-iron diamine alcian blue(HID+AB) PH2.5 and AB pH1.0 staining for dissolving these problems.Negative lesions by the HID+AB pH2.5 staining were significantly included in BCAC, further than the aberrant crypt foci(ACE), that is conventional lesions in rat colon carcinogenesis.Especially, the rate of concordance between negative lesion nest HID+AB pH2.5 staining and BCAC which consist of crypts over 4 was high.Therefore, the negative lesion, which consisted of crypts over 4, was able to seem to become the substitution of BCAC.However, the detection of BCAC in the proximal colon was difficult for the HID+AB pH2.5 staining because of the characteristics of the histological feature of proximal colon and the HLD+AB pH2.5 staining. Thus, we examined the possibility of the application of AB pH1.0 alone staining by which sulfomucin and sialomucin can be simultaneously represented as well as HJD+AB pH2.5 staining.The AB pH1.0 alone staining was able to detect the negative lesions easier than HID+AB pH2.5 staining, and it was also proven that the detection was possible on those in the proximal colon.In conclusion, both HID+AB pH2.5 staining and AB pH1.0 alone staining were detectable the conventional preneoplastic lesions such as BCAC and ACF.In the future, it is necessary to search the mutual relationship among these negative lesions, BCAC and ACF.
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