2003 Fiscal Year Final Research Report Summary
Identification of zoonotic isolates of Blastocystis by using polymorphic genomic DNA
| Project/Area Number |
13670245
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Nara Women's University |
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Principal Investigator |
YOSHIKAWA Hisao Nara Women's University, Dept.of Biological Sci., Associate Professor, 理学部, 助教授 (50191557)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Yuzo Gifu University, Dept.of Parasitology, Professor, 医学部, 教授 (80094580)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Blastocystis / Genome / Polymorphism / Zoonotic / PCR / Primer / Genotype / Identification |
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| Research Abstract |
Since Blastocystis organisms have been widely isolated from a variety animals and extensive genetic and karyotypic heterogeneity have been demonstrated among Blastocystis isolates from humans and animals, it is difficult to clarify whether isolated organisms from animals are identical to or different from human B.hominis. In this study, therefore, we developed several sequenced-tagged site (STS) primers from random amplified polymorphic DNA analysis of known strains of B.hominis. Since the STS primers only amplified the distinct subtypes which correspond to the phylogenetically different clades inferred from the small subunit ribosomal DNA sequences, the STS primers can be used as a tool to type genotypes among polymorphic Blastocystis populations. When various isolates from mammals and birds have examined with PCR amplification by using the STS primers, most of the isolates were amplified with distinct primer, suggesting a zoonotic genotypes of animal isolates. These results indicated that Blastocystis organisms isolated from animals are potential sources of human infections.
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