2002 Fiscal Year Final Research Report Summary
Filamentous bacteriophages associated with recent pandemic strains of Vibrio parahaemolyticus
| Project/Area Number |
13670271
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
IIDA Tetsuya Osaka Univ., Research Institute for Microbial Diseases, Associate Professor, 微生物病研究所, 助教授 (90221746)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Vibrio parahaemolyticus / pandemic / filamentous bacteriophage |
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| Research Abstract |
In a previous study, we reported on a filamentous bacteriophage, f237, that is specifically associated wit the recent pandemic 03:K6 serovar strains of Vibrio parahaemolyticus. In this study, we examined distribution of the phage among clinical V. parahaemolyticus strains and characterized the phage itself. The followings are what we have obtained during this research term.
1. We examined the distribution of f237 among more recent clinical V. parahaemolyticus, and revealed that f237 is associated with not only 03:K6 serovar strains, but also other recent pandemic strains with serovar 04:K68 or 01:KUT. These results suggest that f237 is related to newly emerged pandemic strains of V. parahaemolyticus (Emerg. Infect. Dis. 7: 477-478, 2001).
2. We demonstrated that f237 is integrated into the host bacterial chromosome. Also we precisely determined the integration site of the phage (J. Bacteriol. 184: 4933-4935, 2002. Lancet 361: 743-749, 2003).
3. From the non-pandemic V. parahaemolyticus strains, we isolated two filamentou.s bacteriophages that are related to f237, and determined the whole genome sequences of the phages (manuscript in preparation).
4. We identified a new genetic marker of the pandemic V. parahaemolyticus strains (manuscript in preparation).
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Research Products
(9 results)
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[Publications] Iida, T., Hattori, A., Tagomori, K., Nasu, H., Naim, R., and Honda, T.: "Filamentous phage associated with recent pandemic strains of Vibrio parakaemolyticus"Emerg. Infect. Dis.. 7. 477-478 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Iida, T., Makino, K., Nasu, H., Yokoyama, K., Tagomori, K., Hattori, A., Okuno, T., Shinagawa, H., and Honda, T.: "Filamentous bacteriophages of vibrios are integrated into the diflike site of the host chromosome"J. Bacteriol.. 184. 4933-4935 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Makino, K., Oshima, K., Kurokawa, K., Yokoyama, K., Uda, T., Tagomori, K., Iijima, Y., Najima M., Nakano, M., Yamashita, A., Kubota, Y., Kimura, S., Yasunaga, T., Honda, T., Shinagawa, H., Hattori, M. and Iida, T.: "Genome sequence of Vibrio parahaemolyticus : a pathogenic mechanism distinct from that of V cholerae"Lancet. 361. 743-749 (2003)
Description
「研究成果報告書概要(欧文)」より
