2002 Fiscal Year Final Research Report Summary
Virological and immunological Studies of Epstein-Barr virus on the antibodie negative carriers
| Project/Area Number |
13670298
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Tottori University |
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Principal Investigator |
SAIRENJI Takashi Tottori University, Faculty of Medicine, Department of Biomedical Sciences, Professer, 医学部, 教授 (10117351)
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| Co-Investigator(Kenkyū-buntansha) |
HOSHIKAWA Yoshiko Tottori University, Faculty of Medicine,Department of Biomedical Sciences, Research Associate, 医学部, 助手 (10181489)
SATOH Yukio Tottori University, Faculty of Medicine,Department of Biomedical Sciences, Research Associate, 医学部, 助手 (70144657)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Epstein-Barr virus(EBV) / EBV antibodies / EBV DNA / infectious mononucleosis / EBV antibody-negative carriers / EBV infection in children / PCR法 |
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| Research Abstract |
We demonstrated EBV DNA in peripheral blood mononuclear cells (PBMC) from infants with infectious mononucleosis- (IM) like symptoms. Thirteen (76^%) of the 17 patients did not have EBV antibodies; however EBV DNA was detected in 8 PBMC (62^%) from the 13 seronegative patients by PCR. The 4 patients were retested in 6 to 12 months later. Three patients were still seronegative: however EBV DNA was detected. One patient seroconverted and EBV DNA was still detected. The transcript of EBNA1 was detected in one patient but neither EBNA2 nor LMP2A were detected in all PBMC from the 4 tested patients. Type 1 EBV DNA was detected in 5 PBMC of 7 tested patients, and type 2 EBV DNA was detected in the type 1 positive PBMC of one patient as well. The LL-1β polymorphism that is reported to be one of the immunological factors of EBV seronegativity revealed no difference in IM-like patients. These results indicated that EBV infection occurs in EBV-seronegative IM-like infants; however, the modes of infection are clearly different from IM.
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