2003 Fiscal Year Final Research Report Summary
Development of new Japanese-specific DNA markers (SNP) and application to individualization
| Project/Area Number |
13670423
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Tottori University |
|---|
Principal Investigator |
YUASA Isao Tottori University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00093633)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AGO Kazutoshi Kagoshima University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (20102056)
UMEMATSU Kazuo Yamagata University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10091828)
IRIZAWA Yoshito Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (90112226)
|
|---|
| Project Period (FY) |
2001 – 2003
|
| Keywords | Japanese / DNA polymorphism / SNP / Individualization / nucleotide sequence / mtDNA / Y chromosome |
|---|
| Research Abstract |
To make it possible to identify the ethnic origin of cadavers and forensic materials, we have investigated mtDNA and Y chromosome haplogroups in DNA samples from 700-2000 Japanese, Koreans, Chinese Hans, Mongolians and Germans. When more than 20 SNPs in the coding regions of mtDNA were typed simultaneously by APLP method, haplogroup B2 in mtDNA was found in only Japanese and Koreans. However, its frequency was markedly higher in Japanese than in Koreans. Typing of 29 binary polymorphisms in Y chromosome by RFLP permitted us to classify 867 samples from various populations into 24 haplogroups. Though the heterozygosity values were similar, the distribution of haplogroups and the mean number of pairwise differences among haplogroups were very different. A C-to-T transition found in the SRY gene was unique and observed only in Japanese and Koreans. Haplogroups 4 and 5 of YAP lineage and haplogroup 8 of RPS4Y lineage were identified in Japanese at higher frequencies than in Koreans. When these mtDNA and Y chromosome haplogroups are observed, it is strongly suggested that the cadavers and forensic materials are originated from Japanese.
|
-
-
-
-
-
-
-
-
[Publications] K.Umetsu, M.Tanaka, I.Yuasa, N.Saitou, T.Takayasu, N.Fuku, E.Naito, K.Ago, N.Nakayashiki, A.Miyoshi, S.Kashimura, G.Watanabe, M.Osawa: "Multiplex amplified product-length polymorphism analysis for rapid detection of human mitochondrial DNA variations."Electrophoresis. 22(16). 3533-3538 (2001)
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
