2002 Fiscal Year Final Research Report Summary
Mechanisms of antigen-specific DNA vaccines for allergies
| Project/Area Number |
13670443
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Tohoku University |
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Principal Investigator |
SANO Kunio Department of Respiratory and Infectious Diseases, Graduate School of Medicine, Tohoku Univerdty, Research Associate, 大学院・医学研究科, 助手 (20192601)
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| Project Period (FY) |
2001 – 2002
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| Keywords | B cells / CpG ODNs / Th1 cells / antigen-presenting cells / antigen-specificity |
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| Research Abstract |
APCs initiate T cell-mediated immune responses against foreign Ags. Dendritic cells are professional APCs that play unique roles, including Ag-nonspecific capture, priming of naive T cells and Th1 induction, whereas B cells generally lack these functions. In this study, we uncovered novel aspects of murine B cells as APCs using CpG oligodeoxynucleotides (CpG) conjugated with an Ag. B cells served as efficient APCs independently of surface Igs. This characteristic was underlay by the CpG-mediated Ag uptake and presentation, which was functional only when CpG were covalently conjugated to Ag. The B cells cultured with CpG-conjugated Ag not only enhanced the IFN-γ formation by Th1 cells but also induced Th1 differentiation from unprimed T cells. These natures were in parallel with the increase in the expression of CD40, CD86, and class II molecules on B cells, and the coordinated production of IL-12 by the cells. To our knowledge, this is the first report revealing that B cells share with dendritic cells common intrinsic characteristics, such as the Ag-nonspecific capture and presentation, and the induction of Th1 differentiation from unprimed T cells.
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Research Products
(10 results)
