2002 Fiscal Year Final Research Report Summary
Establishment of liver-directed gene transfer by hepatitis E virus like particle
| Project/Area Number |
13670507
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Mie University |
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Principal Investigator |
IMOTO Ichiro Mie University, Hospial, Associate Professor, 医学部附属病院, 助教授 (90176511)
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| Co-Investigator(Kenkyū-buntansha) |
TAMAKI Shigenori Mie University, Hospial, Research Associate, 医学部附属病院, 助手 (80260602)
ADACHI Yukihiko Mie University, Faculty of Medicine, Professor, 医学部, 教授 (50111026)
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| Project Period (FY) |
2001 – 2002
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| Keywords | HEV / liver / virus-like particle / MRP2 |
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| Research Abstract |
1 : Over expression of a part of open reading frame 2 of HEV in the baculovirus expression system allowed this protein to assemble into virus-like paraticle (VLP) and we successfully generated VLP encapsulated plasmid vectors.
2 : We investigated VLP allowed the plasmid encoding reporter gene to enter the cultured cells using fluorescence detection of green fluorescent protein (GFP). The green fluorescence was detected in human hepatoma HepG2 cells and other cell lines.
3 : We investigated VLP allowed the plasmid to enter hepatocyte by the intravenous injection via mouse tail vein. The green fluorescence was detected in hapatocytes.
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