2001 Fiscal Year Annual Research Report

Barrett上皮の腸上皮化生と胃の腸上皮仮生の比較による前癌病変の分子機構解析

Research Project

Project/Area Number	13670533
Research Institution	Sapporo Medical University
Principal Investigator	遠藤高夫札幌医科大学, 医学部, 助教授 (40191928)
Co-Investigator(Kenkyū-buntansha)	山本博幸札幌医科大学, 医学部, 助手 (40332910)
Keywords	Barrett食道 / ムチン分子 / 腸上皮化生 / 拡大内視鏡診
Research Abstract	Barretts上皮の腸上皮仮生と胃幽門部の腸上皮仮生巣はMUC2コアpeptideの発現は両者に差は認められなかったが,アポムチンA3D4の発現はBarrett上皮に強く発現し,またこの側鎖と考えられる硫酸化糖鎖の発現も同様の傾向を示した.Ki-67による細胞増殖活性はBarrett上皮腸上皮化生=胃不完全型腸上皮化生>胃完全型腸上皮化生であった. Barrett上皮を有する患者30名の拡大内視鏡観察を行い,粘膜微細構造を5型に分類した.点状ならびに直線状のpitはアポムチンMUC5ACならびにMUC6を強く発現し(75%),管状,絨毛状のpitはアポムチンMUC2ならびに硫酸化糖鎖sulfo-Lewis a,シアル酸化糖鎖sialyl Tnを高頻度(90%)に認められた.また長楕円型のpitはMUC5ACとMUC2の両者を混在して発現する傾向を見せた。PCNA labeling indexは絨毛状>管状>長楕円状>直線状・点状pitであった. Methylene blueの吸収能は絨毛状,管状のpitの一部にしか観察されなかった.吸収能の差異は免疫組織学的にCD10(neutral endopeptidase)の発現と一致していた.胃幽門部の拡大内視鏡観察では腸上皮化生巣は管状のpitが多く絨毛状のものは認められなかった.Brrett粘膜のSulfo-Lewis aまたはMUC2陽性をout comeとしたばあいの拡大観察におけるsencitivityは40%,specificityは80%であった.

Research Products

(6 results)

All Other

All Publications (6 results)

[Publications] Takao Endo. et al.: "Classification of Barrett's epithelium by magnifying endoscopy"Gastrointestinal Endoscopy. 55(in press). (2002)
[Publications] Takao Endo et al.: "Mucin phenotype and microsatellite instability in multiple early gastric cancers"Int J Cancer. (in press). (2002)
[Publications] Yamashita, K. et al.: "Epstein-barr virus-associated gastric cancer in a patient with dermatomyositis"Int Med. 40(2). 96-99 (2001)
[Publications] Adachi Y, et al.: "Clinicopathologic and prognostic significance of matrilysin expression at the invasive front in human colorectal cancers"Int J Cancer. 95. 290-294 (2001)
[Publications] Kaneto H, et al.: "Detection of hypermethylation of the p16(INK4A) gene promoter in chronic hepatitis and cirrhosis associated with hepatitis B or C virus"Gut. 48. 372-377 (2001)
[Publications] Yoshida, Y. et al.: "Jejunal carcinoid tumor mimicking leiomyosarcoma : preoperative diagnosis by endoscopic biopsy"J. Gastroenterol.. 36. 39-43 (2001)

2001 Fiscal Year Annual Research Report

Barrett上皮の腸上皮化生と胃の腸上皮仮生の比較による前癌病変の分子機構解析

Principal Investigator

遠藤 高夫 札幌医科大学, 医学部, 助教授 (40191928)

Research Products

[Publications] Takao Endo. et al.: "Classification of Barrett's epithelium by magnifying endoscopy"Gastrointestinal Endoscopy. 55(in press). (2002)

[Publications] Takao Endo et al.: "Mucin phenotype and microsatellite instability in multiple early gastric cancers"Int J Cancer. (in press). (2002)

[Publications] Yamashita, K. et al.: "Epstein-barr virus-associated gastric cancer in a patient with dermatomyositis"Int Med. 40(2). 96-99 (2001)

[Publications] Adachi Y, et al.: "Clinicopathologic and prognostic significance of matrilysin expression at the invasive front in human colorectal cancers"Int J Cancer. 95. 290-294 (2001)

[Publications] Kaneto H, et al.: "Detection of hypermethylation of the p16(INK4A) gene promoter in chronic hepatitis and cirrhosis associated with hepatitis B or C virus"Gut. 48. 372-377 (2001)

[Publications] Yoshida, Y. et al.: "Jejunal carcinoid tumor mimicking leiomyosarcoma : preoperative diagnosis by endoscopic biopsy"J. Gastroenterol.. 36. 39-43 (2001)

遠藤高夫札幌医科大学, 医学部, 助教授 (40191928)