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2004 Fiscal Year Final Research Report Summary

Role of intestinal epithelial tight junction permeability in inflammatory bowel disease

Research Project

Project/Area Number 13670576
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionFukuoka University

Principal Investigator

AOYAGI Kunihiko  Fukuoka Univ., Hospital, Lecturer, 病院, 講師 (00253435)

Co-Investigator(Kenkyū-buntansha) SAKISAKA Shotaro  Fukuoka Univ., Sch.Med., Professor, 医学部, 教授 (90158923)
Project Period (FY) 2001 – 2004
KeywordsInflammatory bowel disease / Tight junction
Research Abstract

It is well known that intestinal permeability is increased in inflammatory bowel diseas(IBD including Crohn's disease and ulcerative colitis.
Various intraluminal substances such as endotoxin and other toxins stimulate mucosal inflammatory cells when they pass through the epithelial barrier. Although intestinal permeability is considered to be important in the pathogenesis of IBD, there remains to be unclear. In our study, tight junction(TJ) barrier function was evaluated using monolayer cell cultures (T84) and rat models (5% dextran sulfate sodium-induced colitis).E ffects of antiinflammatory drugs (ecabet sodium and 5-aminosalicylates) were also identified. The structure and localization of TJ protein and intestinal permeability were determined by immunohistochemistry and transepithelial electric resistance(TER) or extravasation of Evans blue dye, respectively.
In cell culture, interferon gamma disrupted the TJ structure and decreased TER, but ecabet sodium recovered them. In DDS colitis, length of the colon, tissue myeloperoxidase activity, histological inflammation score, permeability were increased, and immunohistochemical localization of TJ protein showed a decreased staining, but 5-aminosalicylates administration ameliorated their changes. These results suggest that drugs for IBD improve the disruption of TJ, leading to act, in part, as an anti-inflammatory property. A new clinical approach targeting intestinal tight junction may be promising in IBD.

  • Research Products

    (15 results)

All 2005 2004 2003 2002 2001 Other

All Journal Article (15 results)

  • [Journal Article] Enteral nutrition as a primary therapy of intestinal lymphangiectasia : Value of elemental diet, and polymeric diet compared with total parenteral nutrition2005

    • Author(s)
      Aoyagi K.et al.
    • Journal Title

      Dig Dis Sci. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Evaluation of gastroduodenal lesions in patients with Crohn's disease and ulcerative colitis2004

    • Author(s)
      Maeda K, Okada M, Seo M, Aoyagi K, Yamaguchi M, Sakisaka S.
    • Journal Title

      Dig Endosc. 16

      Pages: 199-203

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Giant esophageal papilloma.2004

    • Author(s)
      Inomata S, Aoyagi K, Eguchi K, Sakisaka S.
    • Journal Title

      Gastrointest Endosc. 60(3)

      Pages: 430

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Endoscopic removal of a spoon from the stomach with a double-snare and balloon.2003

    • Author(s)
      Aoyagi K.et al.
    • Journal Title

      Gastrointest Endosc. 57(7)

      Pages: 990-991

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mortality and cause of death in Japanese patients with Crohn's disease.2003

    • Author(s)
      Uno H.et al.
    • Journal Title

      Dis Colon Rectum. 57(7)

      Pages: S15-S21

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Endoscopic removal of a spoon from the stomach with a double-snare and balloon.2003

    • Author(s)
      Aoyagi K, Maeda K, Morita I, Eguchi K, Nishimura H, Sakisaka S.
    • Journal Title

      Gastrointest Endosc. 57(7)

      Pages: 990-991

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mortality and cause of death in Japanese patients with Crohn's disease.2003

    • Author(s)
      Uno H, Yao T, Matsui T, Sakurai T, Iida M, Matsumoto T, Aoyagi K, Fuchigami T.
    • Journal Title

      Dis Colon Rectum. 46(10 Suppl)

      Pages: S15-S21

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Short-term efficacy of enteral nutrition in the treatment of active Crohn' s disease : a randomized, controlled trial comparing nutrient formulas.2002

    • Author(s)
      Sakurai T.et al.
    • Journal Title

      J Parenter Enteral Nutr. 26(2)

      Pages: 98-103

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Short-term efficacy of enteral nutrition in the treatment of active Crohn's disease : a randomized, controlled trial comparing nutrient formulas.2002

    • Author(s)
      Sakurai T, Matsui T, Yao T, Takagi Y, Hirai F, Aoyagi K, Okada M.
    • Journal Title

      J Parenter Enteral Nutr. 26(2)

      Pages: 98-103

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intraoperative enteroscopy for Crohn's disease : lesion depiction with reference to predictive value of postoperative recurrence.2001

    • Author(s)
      Esaki M et al.
    • Journal Title

      Surg Endos. 15

      Pages: 455-459

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of diet on changes in small intestinal blood flow following intracolonic administration of indomethacin to rats.2001

    • Author(s)
      Yamamoto C et al.
    • Journal Title

      Dig Dis Sci. 46(1)

      Pages: 200-207

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intraoperative enteroscopy for Crohn's disease : lesion depiction with reference to predictive value of postoperative recurrence.2001

    • Author(s)
      Esaki M, Matsumoto T, Hizawa K, Aoyagi K, Mibu R, Iida M, Fujishima M.
    • Journal Title

      Surg Endosc. 15

      Pages: 455-459

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of diet on changes in small intestinal blood flow following intracolonic administration of indomethacin to rats.2001

    • Author(s)
      Yamamoto C, Kawakubo K, Aoyagi K, Matsumoto T, Iida M, Ibayashi S, Kitazono T, Doi K, Kanamoto K, Fujishima M.
    • Journal Title

      Dig Dis Sci. 46(1)

      Pages: 200-207

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Primary biliary cirrhosis associated with ulcerative colitis.2001

    • Author(s)
      Nakayama M, Tsuji H, Shimono J, Azuma K, Ogata H, Matsumoto T, Aoyagi K, Fujishima M, Iida M.
    • Journal Title

      Fukuoka Igaku Zasshi. 92(10)

      Pages: 354-359

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Enteral nutrition as a primary therapy of intestinal lymphangiectasia : Value of elemental diet, and polymeric diet compared with total parenteral nutrition.

    • Author(s)
      Aoyagi K, Iida M, Matsumoto T, Sakisaka S.
    • Journal Title

      Dig Dig Sci. (in press)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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