2002 Fiscal Year Final Research Report Summary
Development of novel therapeutic options for treating heart failure targeting the myosin light chain
| Project/Area Number |
13670689
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMASHITA Hiroshi University of Tokyo, Graduate School of Medicine, Reseach Associate, 医学部附属病院, 助手 (50323572)
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| Co-Investigator(Kenkyū-buntansha) |
SATA Masataka University of Tokyo, Graduate School of Medicine, Reseach Associate, 医学部附属病院, 助手 (80345214)
SUGIURA Seiryo University of Tokyo, Graduate School of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (10272551)
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| Project Period (FY) |
2001 – 2002
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| Keywords | cardiac myosin / myosin light chain / in vitro motility assay |
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| Research Abstract |
A myosin molecule consists of two heavy chains and two pairs of light chains and converts chemical energy of ATP hydrolysis into mechanical work of muscle contraction. The light chains are located close to the heavy chain head domain which contains ATP- and actin-binding sites essential for the motor function. To study functional roles of the myosin light chains, we purified two cardiac myosins with identical heavy chain and different light chains and compared the motor function of these myosins using in vitro motility assay techniques, where mechanical interaction of actin and myosin was reconstituted in vitro. Although catalytic activity showed no difference, motor function of these myosin molecules showed remarkable difference : the myosin molecules with ventricular-type light chains generated higher average force compared to those with atrial-type light chains and had longer duration of force-generating interaction with actin, suggesting that the myosin light chains may play an important role in converting the chemical energy into mechanical work. The unique nature of the myosin light chain modifying force-generating ability of the molecule without changing ATP hydrolysis rate may provide a clue to novel treatment for heart failure augmenting contractile function without increasing ATP consumption and protecting cardiac muscles from deterioration in energy metabolism.
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[Publications] Yasuda S, Sugiura S, Kobayakawa N, Fujita H, Yamashita H, Katoh K, Saeki Y, Kaneko H, Suda Y, Nagai R, Sugi H: "A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers coupled with a feedback system"Am J Physiol. 281. H144-H1446 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yasuda S, Sugiura S, Kobayakawa N, Fujita H, Yamashita H, Katoh K, Saeki Y, Kaneko H, Suda Y, Nagai R, and Sugi H: "A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers coupled with a feedback system"Am J Physiol. 281. H1442-1446 (2001)
Description
「研究成果報告書概要(欧文)」より
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