2002 Fiscal Year Final Research Report Summary
Investigation of receptor-activated Ca^<2+>-permeable channels in smooth muscle cells
| Project/Area Number |
13670691
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAJIMA Toshiaki The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (50227790)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Smooth muscle / Ca^<2+> permeable channel / Transient receptor potential / endothelin-1 / Ca^<2+>-ion / Non-selective cation channel |
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| Research Abstract |
The contractile agonists such as endothelin-1 and vasopressin increase Ca^<2+> influx via several receptor-activated Ca^<2+> channels (Ca^<2+>-release-activated Ca^<2+> channel (CRAC) and Ca^<2+> permeable nonselective cation channels (I_<CAT>)). The analysis of RT-PCR showed the expression of transient receptor potential (TRP1,2,4) and TRP6. The latter may be related to I_<CAT>, and the former may be related to CRAC. Diacylglycerol produced from the activation of PLC, but not IP_3 and protein kinase C, may be involved in the activation of I_<CAT>. Similar results were obtained in human bronchial smooth muscle cells (Am J Respir Cell Mol Biol, 2000 ; 2002). In addition, we have reported that I_<CAT> plays an important role in forming membrane potential in rabbit coronary arterial cells, and lysophosphatidylcholine (LPC) further activated I_<CAT>. Thus, it is likely that I_<CAT> may play a role in regulating smooth muscle tone under the pathophysiological conditions.
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[Publications] Ji Ma, Toshiaki Nakajima, Haruko Iida, Kuniaki Iwasawa, Kuniko Terasawa, Hitoshi Oonuma, Taisuke Jo, Toshihiro Morita, Hiroyuki Imuta, Jun-ichji Suzuki, Ken Hirose, Yukichi Okuda, Nobuhiko Yamada, Ryozo Nagai, Masao Omata: "Inhibitory effects of ursodeoxycholic acid on the induction of nitric oxide synthaae in vascular smooth muscle cells"Eur J Pharmacol. in press. (2003)
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[Publications] Kuniko Terasawa, Toshiaki Nakajima, Haruko Iida, Kuniaki Iwasawa, Hitoshi Oonuma, Taisuke Jo, Toshihiro Morita, Fumitaka Nakamura, Yoshiharu Fujimori, Teruhiko Toyo-oka, Ryozo Nagai: "Nonselective cation currents regulate membrane potential of rabbit coronary arterial cell : Modulation by lysophosphatidylcholine"Circulation. 106. 3111-3119 (2002)
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[Publications] Y. Okuda, K, Kobayashi, H. Ohmori, H. Sone, S. Suzuki, J Ma, T. Nakajima, N, Yamada, K. Yamashita: "Acute gliclazide administration enhances glucose and ketone body utilization in the perfused hind limb of normal and streptozotocin-diabetic rats"Life Sciences. 71. 647-654 (2002)
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[Publications] Tomie Kawada, Mikio Nakazawa, Sakura Nakauchi, Ken Yamazaki, Ryoichi Shimamoto, Masashi Urabe, Jumi Nakata, Chieko Hemmmi, Fujiko Masui, Toshiaki Nakajima, Jun-ichi Suzuki, John Monahan, Hiroshi Sata, Tomoh Masaki, Keiya Ozawa, Teruhiko Toyo-oka: "Rescue of hereditary form of dilated cardiomyopathy by rAAV-mediated somatic gene therapy : Amelioration of morphological findings, sarcolemmal permeability, cardiac performances, and the prognosis of TO-2 hamsters"PNAS. 99. 901-906 (2002)
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